BMRB Entry 34173
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34173
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Title: NMR solution structure of the external DII domain of Rvb2 from Saccharomyces cerevisiae PubMed: 29569106
Deposition date: 2017-08-24 Original release date: 2018-03-23
Authors: Rouillon, C.; Bragantini, B.; Charpentier, B.; Manival, X.; Quinternet, M.
Citation: Bragantini, B.; Rouillon, C.; Charpentier, B.; Manival, X.; Quinternet, M.. "Structural and interaction analysis of the external DII domains of the yeast AAA+ ATPases Rvb1 and Rvb2" Biomol NMR Assign 12, 243-247 (2018).
Assembly members:
entity_1, polymer, 107 residues, 11845.531 Da.
Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: GPHMETELIEGEVVEIQIDR
SITGGHKQGKLTIKTTDMET
IYELGNKMIDGLTKEKVLAG
DVISIDKASGKITKLGRSFA
RSRDYDAMGADTRFVQCPEG
ELQKRKT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 459 |
| 15N chemical shifts | 109 |
| 1H chemical shifts | 755 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 107 residues - 11845.531 Da.
| 1 | GLY | PRO | HIS | MET | GLU | THR | GLU | LEU | ILE | GLU | ||||
| 2 | GLY | GLU | VAL | VAL | GLU | ILE | GLN | ILE | ASP | ARG | ||||
| 3 | SER | ILE | THR | GLY | GLY | HIS | LYS | GLN | GLY | LYS | ||||
| 4 | LEU | THR | ILE | LYS | THR | THR | ASP | MET | GLU | THR | ||||
| 5 | ILE | TYR | GLU | LEU | GLY | ASN | LYS | MET | ILE | ASP | ||||
| 6 | GLY | LEU | THR | LYS | GLU | LYS | VAL | LEU | ALA | GLY | ||||
| 7 | ASP | VAL | ILE | SER | ILE | ASP | LYS | ALA | SER | GLY | ||||
| 8 | LYS | ILE | THR | LYS | LEU | GLY | ARG | SER | PHE | ALA | ||||
| 9 | ARG | SER | ARG | ASP | TYR | ASP | ALA | MET | GLY | ALA | ||||
| 10 | ASP | THR | ARG | PHE | VAL | GLN | CYS | PRO | GLU | GLY | ||||
| 11 | GLU | LEU | GLN | LYS | ARG | LYS | THR |
Samples:
sample_1: Rvb2DII, [U-13C; U-15N], 1 mM
sample_conditions_1: ionic strength: 150 mM; pH: 6.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA, Keller and Wuthrich - chemical shift assignment
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation
TOPSPIN v3.2, Bruker Biospin - collection
NMR spectrometers:
- Bruker AvanceIII 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts