BMRB Entry 4189
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4189
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Title: Solution Structure of Reduced Horse Heart Cytochrome c
Deposition date: 1998-08-20 Original release date: 2000-03-08
Authors: Banci, L.; Bertini, I.; Huber, J.; Spyroulias, G.; Turano, P.
Citation: Banci, L.; Bertini, I.; Huber, J.; Spyroulias, G.; Turano, P.. "Solution Structure of Reduced Horse Heart Cytochrome C" J. Biol. Inorg. Chem. 4, 21-31 (1999).
Assembly members:
cytochrome c, polymer, 104 residues, Formula weight is not available
HEC, non-polymer, 618.503 Da.
Natural source: Common Name: horse Taxonomy ID: 9796 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Equus caballus
Experimental source: Production method: .
Entity Sequences (FASTA):
cytochrome c: GDVEKGKKIFVQKCAQCHTV
EKGGKHKTGPNLHGLFGRKT
GQAPGFTYTDANKNKGITWK
EETLMEYLENPKKYIPGTKM
IFAGIKKKTEREDLIAYLKK
ATNE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 709 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hh cytc | 1 |
| 2 | heme | 2 |
Entities:
Entity 1, hh cytc 104 residues - Formula weight is not available
| 1 | GLY | ASP | VAL | GLU | LYS | GLY | LYS | LYS | ILE | PHE | ||||
| 2 | VAL | GLN | LYS | CYS | ALA | GLN | CYS | HIS | THR | VAL | ||||
| 3 | GLU | LYS | GLY | GLY | LYS | HIS | LYS | THR | GLY | PRO | ||||
| 4 | ASN | LEU | HIS | GLY | LEU | PHE | GLY | ARG | LYS | THR | ||||
| 5 | GLY | GLN | ALA | PRO | GLY | PHE | THR | TYR | THR | ASP | ||||
| 6 | ALA | ASN | LYS | ASN | LYS | GLY | ILE | THR | TRP | LYS | ||||
| 7 | GLU | GLU | THR | LEU | MET | GLU | TYR | LEU | GLU | ASN | ||||
| 8 | PRO | LYS | LYS | TYR | ILE | PRO | GLY | THR | LYS | MET | ||||
| 9 | ILE | PHE | ALA | GLY | ILE | LYS | LYS | LYS | THR | GLU | ||||
| 10 | ARG | GLU | ASP | LEU | ILE | ALA | TYR | LEU | LYS | LYS | ||||
| 11 | ALA | THR | ASN | GLU |
Entity 2, heme - C34 H34 Fe N4 O4 - 618.503 Da.
| 1 | HEC |
Samples:
sample_one: cytochrome c2.5 – 3.0 mM; phosphate 100 mM
sample_two: cytochrome c2.5 – 3.0 mM; phosphate 100 mM
sample_conditions_one: pH: 7.0; pressure: 1013 mbar; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | sample_one | not available | sample_conditions_one |
| TOCSY | sample_one | not available | sample_conditions_one |
Software:
xwinnmr - Multiplication of raw data by a pure cosine-squared function Fourier Transform Polynomial baseline correction
XEASY - Volume integration of cross-peaks on 2D spectra
NMR spectrometers:
- BRUKER AVANCE 800 MHz
- BRUKER AVANCE 600 MHz
Related Database Links:
| BMRB | 1058 1107 1108 1109 1110 1111 1112 1113 1114 1116 1170 1171 1404 16759 17120 17340 1736 1783 17847 17848 1785 1787 1789 216 220 224 2366 2367 2368 243 244 25640 274 285 286 316 317 336 436 437 438 439 |
| PDB | |
| GB | AAB33495 AAI05398 AAX77008 ABA06541 AEB61027 |
| PRF | 610169A 711086A |
| REF | NP_001039526 NP_001123442 NP_001157486 XP_004007999 XP_004418964 |
| SP | P00004 P62894 P62895 P62896 P68096 |
| TPG | DAA30512 |