BMRB Entry 4254
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4254
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Title: 1H, 15N and 13C Assignments of the DNA Binding Domain of Transcription Factor Mbp1 from Sassharomyces cerevisiae in Both Its Free and DNA Bound Forms and 1H Assignments of the Free DNA
Deposition date: 1998-10-27 Original release date: 1999-10-26
Authors: McIntosh, Pauline; Taylor, Ian; Smerdon, Steven; Frenkiel, Thomas; Lane, Andrew
Citation: McIntosh, Pauline; Taylor, Ian; Smerdon, Steven; Frenkiel, Thomas; Lane, Andrew. "1H, 15N and 13C Assignments of the DNA Binding Domain of Transcription Factor Mbp1 from Saccharomyces cerevisiae in Both Its Free and DNA Bound Forms and 1H Assignments of the Fee DNA" J. Biomol. NMR 13, 397-398 (1999).
Assembly members:
MLU1-BOX binding protein, polymer, 135 residues, 15436.7 Da.
Natural source: Common Name: Baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
MLU1-BOX binding protein: SNQIYSARYSGVDVYEFIHS
TGSIMKRKKDDWVNATHILK
AANFAKAKRTRILEKEVLKE
THEKVQGGFGKYQGTWVPLN
IAKQLAEKFSVYDQLKPLFD
FTQTDGSASPPPAPKHHHAS
KVDKLAAALEHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 242 |
| 15N chemical shifts | 126 |
| 1H chemical shifts | 488 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Mbp1 | 1 |
Entities:
Entity 1, Mbp1 135 residues - 15436.7 Da.
| 1 | SER | ASN | GLN | ILE | TYR | SER | ALA | ARG | TYR | SER | ||||
| 2 | GLY | VAL | ASP | VAL | TYR | GLU | PHE | ILE | HIS | SER | ||||
| 3 | THR | GLY | SER | ILE | MET | LYS | ARG | LYS | LYS | ASP | ||||
| 4 | ASP | TRP | VAL | ASN | ALA | THR | HIS | ILE | LEU | LYS | ||||
| 5 | ALA | ALA | ASN | PHE | ALA | LYS | ALA | LYS | ARG | THR | ||||
| 6 | ARG | ILE | LEU | GLU | LYS | GLU | VAL | LEU | LYS | GLU | ||||
| 7 | THR | HIS | GLU | LYS | VAL | GLN | GLY | GLY | PHE | GLY | ||||
| 8 | LYS | TYR | GLN | GLY | THR | TRP | VAL | PRO | LEU | ASN | ||||
| 9 | ILE | ALA | LYS | GLN | LEU | ALA | GLU | LYS | PHE | SER | ||||
| 10 | VAL | TYR | ASP | GLN | LEU | LYS | PRO | LEU | PHE | ASP | ||||
| 11 | PHE | THR | GLN | THR | ASP | GLY | SER | ALA | SER | PRO | ||||
| 12 | PRO | PRO | ALA | PRO | LYS | HIS | HIS | HIS | ALA | SER | ||||
| 13 | LYS | VAL | ASP | LYS | LEU | ALA | ALA | ALA | LEU | GLU | ||||
| 14 | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_one: MLU1-BOX binding protein, [U-15N], 0.8 – 1.3 mM
sample_two: MLU1-BOX binding protein, [U-15N;U-13C], 0.8 – 1.3 mM
sample_three: MLU1-BOX binding protein0.8 – 1.3 mM
sample_conditions_one: pH: 7.6; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | not available | not available | sample_conditions_one |
Software:
No software information available
NMR spectrometers:
Related Database Links:
| BMRB | 4256 |
| PDB | |
| DBJ | GAA22190 |
| EMBL | CAA52271 CAA98618 CAY78452 |
| GB | AAS64332 AAS64333 AAS64335 AAS64336 AAS64338 |
| REF | NP_010227 |
| SP | P39678 |
| TPG | DAA11800 |
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