BMRB Entry 4417
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4417
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Title: 1H, 13C and 15N Chemical Shift Assignments of the Birch Pollen Allergen Bet v 1
Deposition date: 1999-09-21 Original release date: 2000-01-11
Authors: Schweimer, Kristian; Sticht, Heinrich; Boehm, Markus; Roesch, Paul
Citation: Schweimer, K.; Sticht, H.; Nerkamp, J.; Boehm, M.; Breitenbach, M.; Vieths, S.; Roesch, P.. "NMR Spectroscopy Reveals Common Structural Features of the Birch Pollen Allergen Bet v 1 and the Cherry Allergen Pru a 1" Appl. Magn. Reson. 17, 449-646 (1999).
Assembly members:
Major Pollen Allergen Bet v 1-L, polymer, 159 residues, Formula weight is not available
Natural source: Common Name: White Birch Taxonomy ID: 3505 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Betula verrucosa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Major Pollen Allergen Bet v 1-L: GVFNYETETTSVIPAARLFK
AFILDGDNLFPKVAPQAISS
VENIEGNGGPGTIKKISFPE
GFPFKYVKDRVDEVDHTNFK
YNYSVIEGGPIGDTLEKISN
EIKIVATPDGGSILKISNKY
HTKGDHEVKAEQVKASKELG
ETLLRAVESYLLAHSDAYN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 565 |
| 15N chemical shifts | 152 |
| 1H chemical shifts | 932 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Bet v 1-L | 1 |
Entities:
Entity 1, Bet v 1-L 159 residues - Formula weight is not available
| 1 | GLY | VAL | PHE | ASN | TYR | GLU | THR | GLU | THR | THR | ||||
| 2 | SER | VAL | ILE | PRO | ALA | ALA | ARG | LEU | PHE | LYS | ||||
| 3 | ALA | PHE | ILE | LEU | ASP | GLY | ASP | ASN | LEU | PHE | ||||
| 4 | PRO | LYS | VAL | ALA | PRO | GLN | ALA | ILE | SER | SER | ||||
| 5 | VAL | GLU | ASN | ILE | GLU | GLY | ASN | GLY | GLY | PRO | ||||
| 6 | GLY | THR | ILE | LYS | LYS | ILE | SER | PHE | PRO | GLU | ||||
| 7 | GLY | PHE | PRO | PHE | LYS | TYR | VAL | LYS | ASP | ARG | ||||
| 8 | VAL | ASP | GLU | VAL | ASP | HIS | THR | ASN | PHE | LYS | ||||
| 9 | TYR | ASN | TYR | SER | VAL | ILE | GLU | GLY | GLY | PRO | ||||
| 10 | ILE | GLY | ASP | THR | LEU | GLU | LYS | ILE | SER | ASN | ||||
| 11 | GLU | ILE | LYS | ILE | VAL | ALA | THR | PRO | ASP | GLY | ||||
| 12 | GLY | SER | ILE | LEU | LYS | ILE | SER | ASN | LYS | TYR | ||||
| 13 | HIS | THR | LYS | GLY | ASP | HIS | GLU | VAL | LYS | ALA | ||||
| 14 | GLU | GLN | VAL | LYS | ALA | SER | LYS | GLU | LEU | GLY | ||||
| 15 | GLU | THR | LEU | LEU | ARG | ALA | VAL | GLU | SER | TYR | ||||
| 16 | LEU | LEU | ALA | HIS | SER | ASP | ALA | TYR | ASN |
Samples:
sample_1: Major Pollen Allergen Bet v 1-L, [U-95% 13C; U-95% 15N], 0.7 – 1.1 mM
sample_2: Major Pollen Allergen Bet v 1-L, [U-98% 15N], 1.0 mM
sample_3: Major Pollen Allergen Bet v 1-L 0.8 mM
sample_conditions_1: ionic strength: 10 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D-HNCO | not available | not available | sample_conditions_1 |
| 3D-HNCA | not available | not available | sample_conditions_1 |
| 3D-CBCA(CO)NH | not available | not available | sample_conditions_1 |
| 3D-HNCACB | not available | not available | sample_conditions_1 |
| 3D-HCCH-COSY | not available | not available | sample_conditions_1 |
| 3D-HCCH-TOCSY | not available | not available | sample_conditions_1 |
| 3D-13C-NOESYHSQC | not available | not available | sample_conditions_1 |
| 3D-15N-NOESYHSQC | not available | not available | sample_conditions_1 |
| 3D-15N-TOCSYHSQC, | not available | not available | sample_conditions_1 |
| 3D-HNHA | not available | not available | sample_conditions_1 |
| 2D-NOESY(D2O) | not available | not available | sample_conditions_1 |
| 2D-TOCSY(D2O) | not available | not available | sample_conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB | |
| EMBL | CAA04825 CAA05187 CAA05188 CAA05189 CAA07319 |
| GB | AAD26561 ABC41579 ABC41580 ABC41581 ABC41611 |
| SP | P15494 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts