BMRB Entry 4473
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4473
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Title: The molecular basis for Protein Kinase A anchoring revealed by solution NMR
Deposition date: 1998-12-08 Original release date: 2000-05-25
Authors: Newlon, M.; Roy, M.; Morikis, D.; Hausken, Z.; Coghlan, V.; Scott, J.; Jennings, P.
Citation: NEWLON, M.; ROY, M.; MORIKIS, D.; HAUSKEN, Z.; COGHLAN, V.; SCOTT, J.; JENNINGS, P.. "The Molecular Basis for Protein Kinase A Anchoring Revealed by Solution NMR" Nat. Struct. Biol. 6, 222-227 (1999).
Assembly members:
Camp dependent protein kinase type ii regulatory chain, polymer, 46 residues, Formula weight is not available
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Camp dependent protein kinase type ii regulatory chain: HMGHIQIPPGLTELLQGYTV
EVLRQQPPDLVDFAVEYFTR
LREARR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 305 |
| 13C chemical shifts | 156 |
| 15N chemical shifts | 41 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | chain A | 1 |
| 2 | chain B | 1 |
Entities:
Entity 1, chain A 46 residues - Formula weight is not available
| 1 | HIS | MET | GLY | HIS | ILE | GLN | ILE | PRO | PRO | GLY | ||||
| 2 | LEU | THR | GLU | LEU | LEU | GLN | GLY | TYR | THR | VAL | ||||
| 3 | GLU | VAL | LEU | ARG | GLN | GLN | PRO | PRO | ASP | LEU | ||||
| 4 | VAL | ASP | PHE | ALA | VAL | GLU | TYR | PHE | THR | ARG | ||||
| 5 | LEU | ARG | GLU | ALA | ARG | ARG |
Samples:
sample_1: Camp dependent protein kinase type ii regulatory chain
sample_cond_1: pH: 4.0; temperature: 298 K; ionic strength: 0.012 M; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D NOESY-HSQC | not available | not available | not available |
| 3D TOCSY-HMQC | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNCA,HN(CO)CA | not available | not available | not available |
| 13C-filtered NOESY | not available | not available | not available |
| HNHA | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DMX 500 MHz
Related Database Links:
| PDB | |
| GB | AAM97689 ABK42343 EDL77146 EDL77147 EDL77148 |
| REF | NP_062137 XP_005075011 XP_005347993 XP_006243774 XP_006978360 |
| SP | P12368 |
Download simulated HSQC data in one of the following formats:
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