BMRB Entry 4491

Name: Solution structure of the apo EH1 domain of mouse Eps15
Published: 2000-06-17

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4491

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution structure of the apo EH1 domain of mouse Eps15 PubMed: 9835057

Deposition date: 1999-06-02 Original release date: 2000-06-17

Authors: Whitehead, B.; Tessari, M.; Carotenuto, A.; van Bergen en Henegouwen, P.; Vuister, G.

Citation: Whitehead, B.; Tessari, M.; Versteeg, H.; van Delft, S.; van Bergen en Henegouwen, P.; Vuister, G.. "Sequence-specific 1H, 13C and 15N assignment of the EH1 domain of mouse Eps15" J. Biomol. NMR 12, 465-466 (1998).

Assembly members:
Eps15, polymer, 120 residues, Formula weight is not available

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source: Production method: recombinant technology Host organism: Escherichia Coli

Entity Sequences (FASTA):
Eps15: MAAAAQLSLTQLSSGNPVYE KYYRQVEAGNTGRVLALDAA AFLKKSGLPDLILGKIWDLA DTDGKGVLSKQEFFVALRLV ACAQNGLEVSLSSLSLAVPP PRFHDSSSPLLTSGPSVAEL

Data sets:

assigned_chemical_shifts
- chem_shift_set_1

Data type	Count
13C chemical shifts	501
15N chemical shifts	124
1H chemical shifts	774

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Eps15	1

Entities:

Entity 1, Eps15 120 residues - Formula weight is not available

1	MET	ALA	ALA	ALA	ALA	GLN	LEU	SER	LEU	THR
2	GLN	LEU	SER	SER	GLY	ASN	PRO	VAL	TYR	GLU
3	LYS	TYR	TYR	ARG	GLN	VAL	GLU	ALA	GLY	ASN
4	THR	GLY	ARG	VAL	LEU	ALA	LEU	ASP	ALA	ALA
5	ALA	PHE	LEU	LYS	LYS	SER	GLY	LEU	PRO	ASP
6	LEU	ILE	LEU	GLY	LYS	ILE	TRP	ASP	LEU	ALA
7	ASP	THR	ASP	GLY	LYS	GLY	VAL	LEU	SER	LYS
8	GLN	GLU	PHE	PHE	VAL	ALA	LEU	ARG	LEU	VAL
9	ALA	CYS	ALA	GLN	ASN	GLY	LEU	GLU	VAL	SER
10	LEU	SER	SER	LEU	SER	LEU	ALA	VAL	PRO	PRO
11	PRO	ARG	PHE	HIS	ASP	SER	SER	SER	PRO	LEU
12	LEU	THR	SER	GLY	PRO	SER	VAL	ALA	GLU	LEU

Samples:

sample_one: Eps15, [U-15N], 1.0 mM; potassium phosphate 100 mM; sodium chloride 100 mM; H2O 90%; D2O 10%

sample_two: Eps15, [U-15N; U-13C], 1.0 mM; potassium phosphate 100 mM; sodium chloride 100 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 100 mM; pH: 5.2; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

No software information available

NMR spectrometers:

Varian UnityInova 500 MHz
Varian UnityInova 750 MHz

BMRB	4140
PDB	1QJT

Biological Magnetic Resonance Data Bank