BMRB Entry 4697
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4697
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Title: Structure of the fMet-tRNAfMet-binding domain of B. stearothermophilus initiation factor IF2
Deposition date: 2000-03-22 Original release date: 2006-06-01
Authors: Meunier, Sylvie; Spurio, Roberto; Czisch, Michael; Wechselberger, Rainer; Guennuegues, Marc; Gualerzi, Claudio; Boelens, Rolf
Citation: Meunier, Sylvie; Spurio, Roberto; Czisch, Michael; Wechselberger, Rainer; Guennuegues, Marc; Gualerzi, Claudio; Boelens, Rolf. "Structure of the fMet-tRNA(fMet)-binding domain of B. stearothermophilus initiation factor IF2" EMBO J. 19, 1918-1926 (2000).
Assembly members:
fMet-tRNA binding domain of initiation factor IF2, polymer, 112 residues, Formula weight is not available
Natural source: Common Name: Bacillus stearothermophilus Taxonomy ID: 1422 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus stearothermophilus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
fMet-tRNA binding domain of initiation factor IF2: EIEAAMKGMLDPEYEEKVIG
QAEVRQTFKVSKVGTIAGCY
VTDGKITRDSKVRLIRQGIV
VYEGEIDSLKRYKDDVREVA
QGYECGLTIKNFNDIKEGDV
IEAYVMQEVARA
- assigned_chemical_shifts
- heteronucl_NOEs
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
| Data type | Count |
| 13C chemical shifts | 291 |
| 15N chemical shifts | 122 |
| 1H chemical shifts | 688 |
| heteronuclear NOE values | 90 |
| T1 relaxation values | 90 |
| T2 relaxation values | 90 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | IF2 C-2 | 1 |
Entities:
Entity 1, IF2 C-2 112 residues - Formula weight is not available
| 1 | GLU | ILE | GLU | ALA | ALA | MET | LYS | GLY | MET | LEU | ||||
| 2 | ASP | PRO | GLU | TYR | GLU | GLU | LYS | VAL | ILE | GLY | ||||
| 3 | GLN | ALA | GLU | VAL | ARG | GLN | THR | PHE | LYS | VAL | ||||
| 4 | SER | LYS | VAL | GLY | THR | ILE | ALA | GLY | CYS | TYR | ||||
| 5 | VAL | THR | ASP | GLY | LYS | ILE | THR | ARG | ASP | SER | ||||
| 6 | LYS | VAL | ARG | LEU | ILE | ARG | GLN | GLY | ILE | VAL | ||||
| 7 | VAL | TYR | GLU | GLY | GLU | ILE | ASP | SER | LEU | LYS | ||||
| 8 | ARG | TYR | LYS | ASP | ASP | VAL | ARG | GLU | VAL | ALA | ||||
| 9 | GLN | GLY | TYR | GLU | CYS | GLY | LEU | THR | ILE | LYS | ||||
| 10 | ASN | PHE | ASN | ASP | ILE | LYS | GLU | GLY | ASP | VAL | ||||
| 11 | ILE | GLU | ALA | TYR | VAL | MET | GLN | GLU | VAL | ALA | ||||
| 12 | ARG | ALA |
Samples:
sample_1: fMet-tRNA binding domain of initiation factor IF2, [U-98% 15N], 1.5 mM
sample_2: fMet-tRNA binding domain of initiation factor IF2, [U-10% 13C; U-98% 15N], 1.5 mM
conditions: ionic strength: 0.200 M; pH: 5.2; temperature: 312 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | not available | not available | conditions |
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz
Related Database Links:
| PDB | |
| DBJ | BAD75548 GAD12201 GAJ59418 |
| EMBL | CAA27987 |
| GB | ABO66491 ACX78663 ADI27260 ADU93636 AEV18768 |
| REF | WP_008878544 WP_011230763 WP_011887154 WP_013145729 WP_013523417 |
| SP | A4IMD7 P04766 Q5L0I8 |
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