BMRB Entry 4833
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4833
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Title: 1H, 13C, and 15N assignments for the Antifungal protein from Streptomyces tendae Tu901 PubMed: 11350173
Deposition date: 2000-09-18 Original release date: 2001-07-11
Authors: Campos-Olivas, Ramon; Horr, Ingmar; Bormann, Christiane; Jung, Guenther; Gronenborn, Angela
Citation: Campos-Olivas, Ramon; Horr, Ingmar; Bormann, Christiane; Jung, Guenther; Gronenborn, Angela. "Solution Structure, Backbone Dynamics and Chitin Binding of the Anti-fungal Protein from Streptomyces tendae Tu901" J. Mol. Biol. 308, 765-782 (2001).
Assembly members:
Antifungal protein from Streptomyces tendae Tu901, polymer, 87 residues, 9999.2 Da.
Natural source: Common Name: not available Taxonomy ID: 1932 Superkingdom: Eubacteria Kingdom: not available Genus/species: Streptomyces tendae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Antifungal protein from Streptomyces tendae Tu901: MINRTDCNENSYLEIHNNEG
RDTLCFANAGTMPVAIYGVN
WVESGNNVVTLQFQRNLSDP
RLETITLQKWGSWNPGHIHE
ILSIRIY
Data type | Count |
1H chemical shifts | 597 |
13C chemical shifts | 376 |
15N chemical shifts | 97 |
coupling constants | 68 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | AFP-1 monomer | 1 |
Entities:
Entity 1, AFP-1 monomer 87 residues - 9999.2 Da.
1 | MET | ILE | ASN | ARG | THR | ASP | CYS | ASN | GLU | ASN | ||||
2 | SER | TYR | LEU | GLU | ILE | HIS | ASN | ASN | GLU | GLY | ||||
3 | ARG | ASP | THR | LEU | CYS | PHE | ALA | ASN | ALA | GLY | ||||
4 | THR | MET | PRO | VAL | ALA | ILE | TYR | GLY | VAL | ASN | ||||
5 | TRP | VAL | GLU | SER | GLY | ASN | ASN | VAL | VAL | THR | ||||
6 | LEU | GLN | PHE | GLN | ARG | ASN | LEU | SER | ASP | PRO | ||||
7 | ARG | LEU | GLU | THR | ILE | THR | LEU | GLN | LYS | TRP | ||||
8 | GLY | SER | TRP | ASN | PRO | GLY | HIS | ILE | HIS | GLU | ||||
9 | ILE | LEU | SER | ILE | ARG | ILE | TYR |
Samples:
sample_1: Antifungal protein from Streptomyces tendae Tu901, [U-99% 15N; U-10% 13C], 0.5 0.8 mM; phosphate 20 mM; NaCl 0.1 M; EDTA 20 uM; NaN3 0.01%; D2O 8.3%; H2O 91.7%
sample_2: Antifungal protein from Streptomyces tendae Tu901, [U-99% 15N; U-13C], 0.5 0.8 mM; phosphate 20 mM; NaCl 0.1 M; EDTA 20 uM; NaN3 0.01%; D2O 8.3%; H2O 91.7%
sample_3: Antifungal protein from Streptomyces tendae Tu901, [U-99% 15N; U-10% 13C], 0.5 0.8 mM; phosphate 20 mM; NaCl 0.1 M; EDTA 20 uM; NaN3 0.01%; D2O 100%
sample_4: Antifungal protein from Streptomyces tendae Tu901, [U-99% 15N; U-13C], 0.5 0.8 mM; phosphate 20 mM; NaCl 0.1 M; EDTA 20 uM; NaN3 0.01%; D2O 100%
conditions_1: pH: 8.0; temperature: 293 K; ionic strength: 0.32 M
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H NOESY | not available | not available | not available |
2D 1H-15N HSQC | not available | not available | not available |
2D 1H-13C HSQC/HMQC | not available | not available | not available |
3D HNCO | not available | not available | not available |
3D HNCACB | not available | not available | not available |
3D CBCA(CO)NH | not available | not available | not available |
3D HNHA | not available | not available | not available |
3D HNHB | not available | not available | not available |
3D 15N-edited NOESY | not available | not available | not available |
4D 13C,13C NOESY | not available | not available | not available |
Software:
NMRView v4.1.1. -
NMR spectrometers:
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- Bruker DMX 600 MHz
- Bruker DMX 750 MHz
- Bruker DRX 800 MHz
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