BMRB Entry 4907
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4907
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Title: NMR Solution Structure of Phospholamban
Deposition date: 2000-11-30 Original release date: 2000-12-22
Authors: Lamberth, Stefanie; Schmid, Holger; Muenchbach, Martin; Vorherr, Thomas; Krebs, Joachim; Carafoli, Ernesto; Griesinger, Christian
Citation: Griesinger, Christian. "NMR Solution Structure of Phospholamban" Helv. Chim. Acta 83, 2141-2152 (2000).
Assembly members:
Phospholamban, polymer, 52 residues, Formula weight is not available
Natural source: Common Name: pig Taxonomy ID: 9823 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Sus scrofa
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Phospholamban: MDKVQYLTRSAIRRASTIEM
PQQARQNLQNLFINFCLILI
FLLLICIIVMLL
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 361 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | phospholamban | 1 |
Entities:
Entity 1, phospholamban 52 residues - Formula weight is not available
| 1 | MET | ASP | LYS | VAL | GLN | TYR | LEU | THR | ARG | SER | ||||
| 2 | ALA | ILE | ARG | ARG | ALA | SER | THR | ILE | GLU | MET | ||||
| 3 | PRO | GLN | GLN | ALA | ARG | GLN | ASN | LEU | GLN | ASN | ||||
| 4 | LEU | PHE | ILE | ASN | PHE | CYS | LEU | ILE | LEU | ILE | ||||
| 5 | PHE | LEU | LEU | LEU | ILE | CYS | ILE | ILE | VAL | MET | ||||
| 6 | LEU | LEU |
Samples:
sample_1: Phospholamban 1.0 mM; CDCl3, [U-2H], 50 %(v/v); CD3OH 50 %(v/v)
exp_cond: pH: 7.0; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | not available | not available | not available |
| TOCSY | not available | not available | not available |
| NOESY | not available | not available | not available |
Software:
FELIX v98 -
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DRX 800 MHz
Related Database Links:
| PDB | |
| EMBL | CAA33171 CAA68461 |
| GB | AAA30884 AAC41618 AAT44582 AAT48602 |
| REF | NP_001003332 NP_999378 XP_004011215 XP_005684581 XP_005907763 |
| SP | P61012 P61013 |
| TPG | DAA26325 |