BMRB Entry 5070
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5070
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Title: Structure and dynamics of the anticodon-arm binding domain of Bacillus stearothermophilus tyrosyl-tRNA synthetase
Deposition date: 2001-07-06 Original release date: 2002-03-29
Authors: Guijarro, J.; Pintar, Alessandro; Prochnicka-Chalufour, Ada; Guez, Valerie; Gilquin, Bernard; Bedouelle, Hugues; Delepierre, Muriel
Citation: Guijarro, J.; Pintar, Alessandro; Prochnicka-Chalufour, Ada; Guez, Valerie; Gilquin, Bernard; Bedouelle, Hugues; Delepierre, Muriel. "Structure and dynamics of the anticodon-arm binding domain of Bacillus stearothermophilus tyrosyl-tRNA synthetase" Structure 10, 311-317 (2002).
Assembly members:
TyrRS C-terminal domain, polymer, 107 residues, 11980.57 Da.
Natural source: Common Name: not available Taxonomy ID: 1422 Superkingdom: Eubacteria Kingdom: not available Genus/species: Bacillus stearothermophilus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
TyrRS C-terminal domain: ALFSGDIANLTAAEIEQGFK
DVPSFVHEGGDVPLVELLVS
AGISPSKRQAREDIQNGAIY
VNGERLQDVGAILTAEHRLE
GRFTVIRRGKKKYYLIRYAL
EHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 666 |
| 13C chemical shifts | 368 |
| 15N chemical shifts | 101 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TyrRS C-ter. domain | 1 |
Entities:
Entity 1, TyrRS C-ter. domain 107 residues - 11980.57 Da.
| 1 | ALA | LEU | PHE | SER | GLY | ASP | ILE | ALA | ASN | LEU | ||||
| 2 | THR | ALA | ALA | GLU | ILE | GLU | GLN | GLY | PHE | LYS | ||||
| 3 | ASP | VAL | PRO | SER | PHE | VAL | HIS | GLU | GLY | GLY | ||||
| 4 | ASP | VAL | PRO | LEU | VAL | GLU | LEU | LEU | VAL | SER | ||||
| 5 | ALA | GLY | ILE | SER | PRO | SER | LYS | ARG | GLN | ALA | ||||
| 6 | ARG | GLU | ASP | ILE | GLN | ASN | GLY | ALA | ILE | TYR | ||||
| 7 | VAL | ASN | GLY | GLU | ARG | LEU | GLN | ASP | VAL | GLY | ||||
| 8 | ALA | ILE | LEU | THR | ALA | GLU | HIS | ARG | LEU | GLU | ||||
| 9 | GLY | ARG | PHE | THR | VAL | ILE | ARG | ARG | GLY | LYS | ||||
| 10 | LYS | LYS | TYR | TYR | LEU | ILE | ARG | TYR | ALA | LEU | ||||
| 11 | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: TyrRS C-terminal domain, [U-15N], 1.2 mM; potassium phosphate 20 mM; H2O 90%; D2O 10%
sample_2: TyrRS C-terminal domain, [U-13C; U-15N], 0.8 mM; potassium phosphate 20 mM; H2O 90%; D2O 10%
Cond_set_1: pH: 6.8; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | not available | not available | not available |
| HNCO | not available | not available | not available |
| 1H-15N HSQC | not available | not available | not available |
| 1H-13C HSQC | not available | not available | not available |
| 15N-edited NOESY-HSQC | not available | not available | not available |
| TOCSY-HSQC | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| H(CC-TOCSY)NNH | not available | not available | not available |
| C(CC-TOCSY)NNH | not available | not available | not available |
| 13C-edited HCCH-TOCSY | not available | not available | not available |
Software:
VNMR v6.1B - acquisition, data processing
XEASY v1.2 - Assignment
NMR spectrometers:
- Varian INOVA 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAD77088 GAD11882 GAJ57796 |
| GB | AAA22877 ACX77385 ADI25737 ADU95213 AEV20456 |
| REF | WP_011232277 WP_013144460 WP_013524353 WP_014196563 WP_015375638 |
| SP | P00952 P04077 Q5KW48 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts