BMRB Entry 5157
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5157
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Title: Solution structure of a hydrophobic analogue of the winter flounder antifreeze protein PubMed: 11856360
Deposition date: 2001-09-24 Original release date: 2008-07-16
Authors: Liepinsh, Edvards; Otting, Gottfried; Harding, Margaret; Ward, Leanne; Mackay, Joel; Haymet, A.
Citation: Liepinsh, Edvards; Otting, Gottfried; Harding, Margaret; Ward, Leanne; Mackay, Joel; Haymet, A.. "Solution Structure of a Hydrophobic Analogue of the Winter Flounder Antifreeze Protein " Eur. J. Biochem. 269, 1259-1266 (2002).
Assembly members:
antifreeze protein type 1 mutant, polymer, 37 residues, 3464 Da.
Natural source: Common Name: winter flounder Taxonomy ID: 8265 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Pseudopleuronectes americanus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
antifreeze protein type 1 mutant: DVASDAKAAAELVAANAKAA
AELVAANAKAAAEAVAX
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 161 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | type 1 AFP mutant | 1 |
Entities:
Entity 1, type 1 AFP mutant 37 residues - 3464 Da.
| 1 | ASP | VAL | ALA | SER | ASP | ALA | LYS | ALA | ALA | ALA | ||||
| 2 | GLU | LEU | VAL | ALA | ALA | ASN | ALA | LYS | ALA | ALA | ||||
| 3 | ALA | GLU | LEU | VAL | ALA | ALA | ASN | ALA | LYS | ALA | ||||
| 4 | ALA | ALA | GLU | ALA | VAL | ALA | AAR |
Samples:
sample_1: antifreeze protein type 1 mutant 11 mM; NaCl 100 mM
sample_2: antifreeze protein type 1 mutant 2 mM; NaCl 100 mM
ex-cond_1: pH: 4.9; temperature: 288 K; ionic strength: 0.1 M
ex-cond_2: pH: 7.0; temperature: 283 K; ionic strength: 0.1 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | not available |
| 2D ROESY | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
| 2D DQF-COSY | not available | not available | not available |
Software:
PROSA v2.6 - spectra processing
XEASY v2.5 - spectral analysis
DYANA v2.6 - structure calculation
OPAL v2.2 - structure refinement
NMR spectrometers:
- Bruker DMX 600 MHz
- Varian Unity-INOVA 800 MHz
Related Database Links:
| PDB |