BMRB Entry 5338
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5338
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Integrin EGF-like module 3 from the beta-2 subunit PubMed: 11896403
Deposition date: 2002-04-03 Original release date: 2002-04-08
Authors: Beglova, N.; Blacklow, S.; Takagi, J.; Springer, T.
Citation: Beglova, N.; Blacklow, S.; Takagi, J.; Springer, T.. "Cysteine-rich module structure reveals a fulcrum for integrin rearrangement upon activation" Nat. Struct. Biol. 9, 282-287 (2002).
Assembly members:
integrin, polymer, 41 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
integrin: ECDTINCERYNGQVCGGPGR
GLCFCGKCRCHPGFEGSACQ
A
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 42 |
| 1H chemical shifts | 232 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | IEGF-3 | 1 |
Entities:
Entity 1, IEGF-3 41 residues - Formula weight is not available
| 1 | GLU | CYS | ASP | THR | ILE | ASN | CYS | GLU | ARG | TYR | ||||
| 2 | ASN | GLY | GLN | VAL | CYS | GLY | GLY | PRO | GLY | ARG | ||||
| 3 | GLY | LEU | CYS | PHE | CYS | GLY | LYS | CYS | ARG | CYS | ||||
| 4 | HIS | PRO | GLY | PHE | GLU | GLY | SER | ALA | CYS | GLN | ||||
| 5 | ALA |
Samples:
sample_1: integrin, [U-15N], 1 mM; NaPO4 5 mM; H2O 90%; D2O 10%
sample_2: integrin 1 mM; NaPO4 5 mM; D2O 100%
sample_cond_1: ionic strength: 5 mM; pH: 6.2; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | not available | not available | not available |
| 3D 15N-separated TOCSY | not available | not available | not available |
| HMQC-J | not available | not available | not available |
| 1H-NOESY | not available | not available | not available |
| 1H-TOCSY | not available | not available | not available |
| DQF-COSY | not available | not available | not available |
Software:
GIFA v4.3 - processing
XEASY v1.3.13 - data analysis
CNS v1.0 - structure solution
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAD96225 BAG53190 BAG64523 |
| EMBL | CAA45427 CAA68266 CAB90553 |
| GenBank | AAA59490 AAH05861 AAH21077 AAP88748 AAQ14924 |
| REF | NP_000202 NP_001029294 NP_001120963 |
| SWISS-PROT | P05107 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts