BMRB Entry 5465
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5465
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Title: NMR solution structure of human Saposin C PubMed: 14674747
Deposition date: 2002-07-16 Original release date: 2004-03-15
Authors: de Alba, E.; Weiler, S.; Tjandra, N.
Citation: de Alba, E.; Weiler, S.; Tjandra, N.. "Solution structure of human saposin C: pH-dependent interaction with phospholipid vesicles." Biochemistry 42, 14729-14740 (2003).
Assembly members:
SAPOSIN C, polymer, 84 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
SAPOSIN C: SDVYCEVCEFLVKEVTKLID
NNKTEKEILDAFDKMCSKLP
KSLSEECQEVVDTYGSSILS
ILLEEVSPELVCSMLHLCSG
LVPR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 380 |
| 15N chemical shifts | 82 |
| 1H chemical shifts | 610 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SAPOSIN C | 1 |
Entities:
Entity 1, SAPOSIN C 84 residues - Formula weight is not available
| 1 | SER | ASP | VAL | TYR | CYS | GLU | VAL | CYS | GLU | PHE | ||||
| 2 | LEU | VAL | LYS | GLU | VAL | THR | LYS | LEU | ILE | ASP | ||||
| 3 | ASN | ASN | LYS | THR | GLU | LYS | GLU | ILE | LEU | ASP | ||||
| 4 | ALA | PHE | ASP | LYS | MET | CYS | SER | LYS | LEU | PRO | ||||
| 5 | LYS | SER | LEU | SER | GLU | GLU | CYS | GLN | GLU | VAL | ||||
| 6 | VAL | ASP | THR | TYR | GLY | SER | SER | ILE | LEU | SER | ||||
| 7 | ILE | LEU | LEU | GLU | GLU | VAL | SER | PRO | GLU | LEU | ||||
| 8 | VAL | CYS | SER | MET | LEU | HIS | LEU | CYS | SER | GLY | ||||
| 9 | LEU | VAL | PRO | ARG |
Samples:
sample_1: SAPOSIN C, [U-15N], 1 mM; NaCl10 – 20 mM; H20 90%; D20 10%
sample_2: SAPOSIN C, [U-13C; U-15N], 1 mM; NaCl10 – 20 mM; H20 90%; D20 10%
sample_3: SAPOSIN C, [U-13C; U-15N], 1 mM; NaCl10 – 20 mM; D20 100%
sample_cond_1: ionic strength: 15 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | not available | not available | not available |
| 4D 13C/15N-separated NOESY | not available | not available | not available |
| 4D 13C-separated NOESY | not available | not available | not available |
| 3D 13C-separated TOCSY | not available | not available | not available |
| CBCACONH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| experiments for residual dipolar coupling measurements | not available | not available | not available |
Software:
xwinnmr v2.5 - collection
NMRPipe v2.1 - processing
PIPP v4.2.8 - data analysis
X-PLOR v3.840 - refinement
NMR spectrometers:
- Bruker DRX 800 MHz
- Bruker DMX 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts