BMRB Entry 5482
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5482
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Assignment of 1H, 13C and 15N resonances of the N-terminal microtubule-binding domain of human doublecortin
Deposition date: 2002-07-29 Original release date: 2003-01-14
Authors: Cierpicki, Tomasz; Kim, Myung Hee; Otlewski, Jacek; Derewenda, Zygmunt; Bushweller, John
Citation: Cierpicki, Tomasz; Kim, Myung Hee; Otlewski, Jacek; Derewenda, Zygmunt; Bushweller, John. "Letter to the Editor: Assignment of 1H, 13C and 15N resonances of the N-terminal microtubule-binding domain of human doublecortin" J. Biomol. NMR 25, 81-82 (2003).
Assembly members:
doublecortin, polymer, 113 residues, 12000 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
doublecortin: GAMDPEFALSNEKKAKKVRF
YRNGDRYFKGIVYAVSSDRF
RSFDALLADLTRSLSDNINL
PQGVRYIYTIDGSRKIGSMD
ELEEGESYVCSSDNFFKKVE
YTKNVNPNWSVNV
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 793 |
| 13C chemical shifts | 353 |
| 15N chemical shifts | 122 |
| coupling constants | 94 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DC 45-150 | 1 |
Entities:
Entity 1, DC 45-150 113 residues - 12000 Da.
| 1 | GLY | ALA | MET | ASP | PRO | GLU | PHE | ALA | LEU | SER | ||||
| 2 | ASN | GLU | LYS | LYS | ALA | LYS | LYS | VAL | ARG | PHE | ||||
| 3 | TYR | ARG | ASN | GLY | ASP | ARG | TYR | PHE | LYS | GLY | ||||
| 4 | ILE | VAL | TYR | ALA | VAL | SER | SER | ASP | ARG | PHE | ||||
| 5 | ARG | SER | PHE | ASP | ALA | LEU | LEU | ALA | ASP | LEU | ||||
| 6 | THR | ARG | SER | LEU | SER | ASP | ASN | ILE | ASN | LEU | ||||
| 7 | PRO | GLN | GLY | VAL | ARG | TYR | ILE | TYR | THR | ILE | ||||
| 8 | ASP | GLY | SER | ARG | LYS | ILE | GLY | SER | MET | ASP | ||||
| 9 | GLU | LEU | GLU | GLU | GLY | GLU | SER | TYR | VAL | CYS | ||||
| 10 | SER | SER | ASP | ASN | PHE | PHE | LYS | LYS | VAL | GLU | ||||
| 11 | TYR | THR | LYS | ASN | VAL | ASN | PRO | ASN | TRP | SER | ||||
| 12 | VAL | ASN | VAL |
Samples:
sample_1: doublecortin, [U-13C; U-15N], 1.0 mM
sample_2: doublecortin, [U-15N], 1.0 mM
DC_cond_1: pH: 6.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | DC_cond_1 |
| 1H-15N TOCSY | not available | not available | DC_cond_1 |
| HNCO | not available | not available | DC_cond_1 |
| HNCACB | not available | not available | DC_cond_1 |
| CBCA(CO)NH | not available | not available | DC_cond_1 |
| CC(CO)NH-TOCSY | not available | not available | DC_cond_1 |
| HC(CO)NH-TOCSY | not available | not available | DC_cond_1 |
| HNHA | not available | not available | DC_cond_1 |
| HCCH-TOCSY | not available | not available | DC_cond_1 |
| HACAHB | not available | not available | DC_cond_1 |
| 15N NOESY | not available | not available | DC_cond_1 |
| 13C NOESY | not available | not available | DC_cond_1 |
Software:
NMRPipe v2.1 - spectral processing
Sparky v3.100 - assignment and analysis
NMR spectrometers:
- Varian INOVA 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAA33387 BAB29230 BAC33328 BAE37637 BAE43338 |
| EMBL | CAA05867 CAA06617 CAC38099 |
| GB | AAC31696 AAC31797 AAC31799 AAC52037 AAG18479 |
| REF | NP_000546 NP_001103692 NP_001103693 NP_001103694 NP_001182482 |
| SP | O43602 O88809 Q9ESI7 |
| TPG | DAA13014 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts