BMRB Entry 5485
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5485
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Title: Thermal stability and solution structure of the ribosomal protein L30e from hyperthermophile archaeon Thermococcus celer PubMed: 12824494
Deposition date: 2002-08-01 Original release date: 2003-07-30
Authors: Chan, Siu-Hong; Leung, Tak-Yuen; Chen, Yu Wai; Bycroft, Mark; Wong, Kam-Bo
Citation: Wong, Kam-Bo; Lee, Chi-Fung; Chan, Siu-Hong; Leung, Tak-Yuen; Chen, Yu Wai; Bycroft, Mark. "Solution Structure and Thermal Stability of Ribosomal Protein L30e from Hyperthermophilic Archaeon Thermococcus celer" Protein Sci. 12, 1483-1495 (2003).
Assembly members:
Ribosomal protein L30e, polymer, 100 residues, Formula weight is not available
Natural source: Common Name: T celer Taxonomy ID: 2264 Superkingdom: Archaea Kingdom: not available Genus/species: Thermococcus celer
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Ribosomal protein L30e: VDFAFELRKAQDTGKIVMGA
RKSIQYAKMGGAKLIIVARN
ARPDIKEDIEYYARLSGIPV
YEFEGTSVELGTLLGRPHTV
SALAVVDPGESRILALGGKE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 700 |
| 13C chemical shifts | 300 |
| 15N chemical shifts | 96 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | L30e | 1 |
Entities:
Entity 1, L30e 100 residues - Formula weight is not available
| 1 | VAL | ASP | PHE | ALA | PHE | GLU | LEU | ARG | LYS | ALA | |
| 2 | GLN | ASP | THR | GLY | LYS | ILE | VAL | MET | GLY | ALA | |
| 3 | ARG | LYS | SER | ILE | GLN | TYR | ALA | LYS | MET | GLY | |
| 4 | GLY | ALA | LYS | LEU | ILE | ILE | VAL | ALA | ARG | ASN | |
| 5 | ALA | ARG | PRO | ASP | ILE | LYS | GLU | ASP | ILE | GLU | |
| 6 | TYR | TYR | ALA | ARG | LEU | SER | GLY | ILE | PRO | VAL | |
| 7 | TYR | GLU | PHE | GLU | GLY | THR | SER | VAL | GLU | LEU | |
| 8 | GLY | THR | LEU | LEU | GLY | ARG | PRO | HIS | THR | VAL | |
| 9 | SER | ALA | LEU | ALA | VAL | VAL | ASP | PRO | GLY | GLU | |
| 10 | SER | ARG | ILE | LEU | ALA | LEU | GLY | GLY | LYS | GLU |
Samples:
sample_1: Ribosomal protein L30e 1 mM; D20 100%
sample_2: Ribosomal protein L30e, [U-100% 15N], 1 mM
sample_3: Ribosomal protein L30e, [U-100% 15N; U-100% 13C], 1 mM
sample_4: Ribosomal protein L30e, [U-100% 15N; U-100% 13C], 1 mM; D20 100%
conditions_1: pH: 5.6; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D TOCSY | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
| 1H-15N HSQC | not available | not available | not available |
| NOESY-HSQC | not available | not available | not available |
| TOCSY-HSQC | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNCA | not available | not available | not available |
| 1H-13C HCCH-TOCSY | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker AMX 500 MHz
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