BMRB Entry 5545
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5545
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Title: NMR assignment of the C-terminal domain of insulin-like growth factor binding protein-6 (IGFBP-6)
Deposition date: 2002-10-02 Original release date: 2003-03-18
Authors: Headey, Stephen; Yao, Shenggen; Parker, Nigel; Kantharidis, Phillip; Bach, Leon; Norton, Raymond
Citation: Headey, Stephen; Yao, Shenggen; Parker, Nigel; Kantharidis, Phillip; Bach, Leon; Norton, Raymond. "Letter to the Editor: 1H, 13C and 15N resonance assignments of the C-terminal domain of insulin-like growth factor binding protein-6 (IGFBP-6)" J. Biomol. NMR 25, 251-252 (2003).
Assembly members:
insulin-like growth factor binding protein-6, polymer, 107 residues, 12056 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
insulin-like growth factor binding protein-6: SYYHHHHHHDYDIPTTENLY
FQGAMGSGPCRRHLDSVLQQ
LQTEVYRGAQTLYVPNCDHR
GFYRKRQCRSSQGQRRGPCW
CVDRMGKSLPGSPDGNGSSS
CPTGSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 366 |
| 15N chemical shifts | 98 |
| 1H chemical shifts | 597 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 161-240 IGFBP-6 | 1 |
Entities:
Entity 1, 161-240 IGFBP-6 107 residues - 12056 Da.
| 1 | SER | TYR | TYR | HIS | HIS | HIS | HIS | HIS | HIS | ASP | ||||
| 2 | TYR | ASP | ILE | PRO | THR | THR | GLU | ASN | LEU | TYR | ||||
| 3 | PHE | GLN | GLY | ALA | MET | GLY | SER | GLY | PRO | CYS | ||||
| 4 | ARG | ARG | HIS | LEU | ASP | SER | VAL | LEU | GLN | GLN | ||||
| 5 | LEU | GLN | THR | GLU | VAL | TYR | ARG | GLY | ALA | GLN | ||||
| 6 | THR | LEU | TYR | VAL | PRO | ASN | CYS | ASP | HIS | ARG | ||||
| 7 | GLY | PHE | TYR | ARG | LYS | ARG | GLN | CYS | ARG | SER | ||||
| 8 | SER | GLN | GLY | GLN | ARG | ARG | GLY | PRO | CYS | TRP | ||||
| 9 | CYS | VAL | ASP | ARG | MET | GLY | LYS | SER | LEU | PRO | ||||
| 10 | GLY | SER | PRO | ASP | GLY | ASN | GLY | SER | SER | SER | ||||
| 11 | CYS | PRO | THR | GLY | SER | SER | GLY |
Samples:
single-labelled_sample: insulin-like growth factor binding protein-6, [U-50% 15N], 1.0 mM; Na acetate 10 mM; azide 0.02%; D2O 5%
double-labelled_sample: insulin-like growth factor binding protein-6, [U-95% 13C; U-50% 15N], 1.0 mM; Na acetate 10 mM; azide 0.02%; D2O 5%
Ex-cond_1: ionic strength: 0.01 M; pH: 4.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY-HSQC | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HNCO | not available | not available | not available |
| HN(CA)CO | not available | not available | not available |
| HNHA | not available | not available | not available |
| HAHB(CO)NH | not available | not available | not available |
| 1H-13C NOESY | not available | not available | not available |
Software:
XWINNMR v3.1 -
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAG35976 |
| EMBL | CAA07346 CAG46590 |
| GenBank | AAA88070 AAB06187 AAH03507 AAH05007 AAH10162 |
| REF | NP_002169 XP_001087498 XP_509089 |
| SWISS-PROT | P24592 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts