BMRB Entry 5614
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5614
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Title: Sheared A(anti)-A(anti) Pairs in a Destabilizing 2x2 Internal Loop: The NMR Structure of 5'(rGGCAAGCCU)2 PubMed: 12475246
Deposition date: 2002-12-03 Original release date: 2002-12-27
Authors: Znosko, B.; Burkard, M.; Schroeder, S.; Krugh, T.; Turner, D.
Citation: Znosko, B.; Burkard, M.; Schroeder, S.; Krugh, T.; Turner, D.. "Sheared A(anti)*A(anti) base Pairs in a Destabilizing 2 x 2 Internal Loop: The NMR Structure of 5'(rGGCAAGCCU)(2)" Biochemistry 41, 14969-14977 (2002).
Assembly members:
dlRNA, polymer, 9 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
dlRNA: GGCAAGCCU
- assigned_chemical_shifts
| Data type | Count |
| 31P chemical shifts | 8 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 5'-R(*GP*GP*CP*AP*AP*GP*CP*CP*U)-3 | 1 |
| 2 | 5'-R(*CP*CP*GP*UP*UP*CP*GP*GP*A)-3 | 1 |
Entities:
Entity 1, 5'-R(*GP*GP*CP*AP*AP*GP*CP*CP*U)-3 9 residues - Formula weight is not available
| 1 | G | G | C | A | A | G | C | C | U |
Samples:
sample_1: dlRNA 1 mM; NaCl 80 mM; phosphate buffer 10 mM; EDTA 0.5 mM; H2O 90%; D2O 10%
sample_2: dlRNA 2 mM; NaCl 80 mM; phosphate buffer 10 mM; EDTA 0.5 mM; D2O 99.996%; D2O 10%
sample_cond_1: ionic strength: 80 mM; pH: 6.8; pressure: 1 atm; temperature: 273 K
sample_cond_2: ionic strength: 80 mM; pH: 6.9; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | not available |
Software:
VNMR v5.2 - collection
FELIX v2000 - data analysis
DISCOVER v95.0 - structure solution
NMR spectrometers:
- Varian INOVA 500 MHz