BMRB Entry 5622
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5622
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Title: Structural and Functional Characterization of a Thioredoxin-like Protein from methanobacterium thermoautotrophicum (Delta H) PubMed: 12834352
Deposition date: 2002-12-11 Original release date: 2003-08-08
Authors: Amegbey, Godwin; Monzavi, Hassan; Habibi-Nazad, Bahram; Bhattacharyya, Sudeepa; Wishart, David
Citation: Amegbey, Godwin; Monzavi, Hassan; Habibi-Nazhad, Bahram; Bhattacharyya, Sudeepa; Wishart, David. "Structural and Functional Characterization of a Thioredoxin-like Protein (Mt0807) from Methanobacterium thermoautotrophicum" Biochemistry 42, 8001-8010 (2003).
Assembly members:
Thioredoxin from M. thermoautotrophicum, polymer, 85 residues, 9481 Da.
Natural source: Common Name: Methanothermobacter thermoautotrophicus Taxonomy ID: 145262 Superkingdom: Archaea Kingdom: Euryarchaeota Genus/species: Methanobacterium thermoautotrophicum
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Thioredoxin from M. thermoautotrophicum: MVVNIEVFTSPTCPYCPMAI
EVVDEAKKEFGDKIDVEKID
IMVDREKAIEYGLMAVPAIA
INGVVRFVGAPSREELFEAI
NDEME
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 582 |
| 13C chemical shifts | 241 |
| 15N chemical shifts | 80 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Mt0807 | 1 |
Entities:
Entity 1, Mt0807 85 residues - 9481 Da.
| 1 | MET | VAL | VAL | ASN | ILE | GLU | VAL | PHE | THR | SER | ||||
| 2 | PRO | THR | CYS | PRO | TYR | CYS | PRO | MET | ALA | ILE | ||||
| 3 | GLU | VAL | VAL | ASP | GLU | ALA | LYS | LYS | GLU | PHE | ||||
| 4 | GLY | ASP | LYS | ILE | ASP | VAL | GLU | LYS | ILE | ASP | ||||
| 5 | ILE | MET | VAL | ASP | ARG | GLU | LYS | ALA | ILE | GLU | ||||
| 6 | TYR | GLY | LEU | MET | ALA | VAL | PRO | ALA | ILE | ALA | ||||
| 7 | ILE | ASN | GLY | VAL | VAL | ARG | PHE | VAL | GLY | ALA | ||||
| 8 | PRO | SER | ARG | GLU | GLU | LEU | PHE | GLU | ALA | ILE | ||||
| 9 | ASN | ASP | GLU | MET | GLU |
Samples:
sample_1: Thioredoxin from M. thermoautotrophicum, [U-13C; U-15N], 1 mM; NaH2PO4 50 mM; NaCl 100 mM
Sample_conditions_1: pH: 6.0; temperature: 298 K; ionic strength: 0.15 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-NOESY | sample_1 | not available | Sample_conditions_1 |
| 2D 1H-TOCSY | sample_1 | not available | Sample_conditions_1 |
| 1H-15N HSQC | sample_1 | not available | Sample_conditions_1 |
| 3D 1H-15N NOESY HSQC | sample_1 | not available | Sample_conditions_1 |
| 3D 1H-15N TOCSY-HSQC | sample_1 | not available | Sample_conditions_1 |
| HNCO | sample_1 | not available | Sample_conditions_1 |
| HNCACB | sample_1 | not available | Sample_conditions_1 |
Software:
VNMR v6.1C -
NMR spectrometers:
- Varian Inova 500 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts