BMRB Entry 5690
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5690
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Title: 1H, 13C, and 15N Chemical Shift Assignments for Cobra Neurotoxin II
Deposition date: 2003-02-11 Original release date: 2003-05-08
Authors: Bocharov, Eduard; Arseniev, Alexandre
Citation: Lyukmanova, Ekaterina; Bocharov, Eduard; Ermolyuk, Yaroslav; Schulga, Alexey; Pluzhnikov, Kirill; Dolgikh, Dmitry; Arseniev, Alexander; Kirpichnikov, Mikhail. "Bacterial expession of isotope labeled snake neurotoxin, disulfide-rich protein" . ., .-..
Assembly members:
neurotoxin II, polymer, 61 residues, Formula weight is not available
Natural source: Common Name: Central Asian cobra Taxonomy ID: 8657 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Naja oxiana
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
neurotoxin II: LECHNQQSSQPPTTKTCSGE
TNCYKKWWSDHRGTIIERGC
GCPKVKPGVNLNCCRTDRCN
N
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 400 |
| 13C chemical shifts | 254 |
| 15N chemical shifts | 71 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | neurotoxin II, nt2 | 1 |
Entities:
Entity 1, neurotoxin II, nt2 61 residues - Formula weight is not available
| 1 | LEU | GLU | CYS | HIS | ASN | GLN | GLN | SER | SER | GLN | ||||
| 2 | PRO | PRO | THR | THR | LYS | THR | CYS | SER | GLY | GLU | ||||
| 3 | THR | ASN | CYS | TYR | LYS | LYS | TRP | TRP | SER | ASP | ||||
| 4 | HIS | ARG | GLY | THR | ILE | ILE | GLU | ARG | GLY | CYS | ||||
| 5 | GLY | CYS | PRO | LYS | VAL | LYS | PRO | GLY | VAL | ASN | ||||
| 6 | LEU | ASN | CYS | CYS | ARG | THR | ASP | ARG | CYS | ASN | ||||
| 7 | ASN |
Samples:
sample_1: neurotoxin II, [U-95% 13C; U-95% 15N], 2 mM
Ex-cond_1: pH: 3.0; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N-HSQC | sample_1 | not available | Ex-cond_1 |
| 13C-HSQC | sample_1 | not available | Ex-cond_1 |
| HNCA | sample_1 | not available | Ex-cond_1 |
| HNCO | sample_1 | not available | Ex-cond_1 |
| HCCH-TOCSY | sample_1 | not available | Ex-cond_1 |
Software:
XEASY v1.2.11 - peak assignments
NMR spectrometers:
- VARIAN UNITY 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts