BMRB Entry 5729
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5729
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Title: Solution Structure, Dynamics and Thermodynamics of the Native State Ensemble of the Sem-5 C-terminal SH3 Domain PubMed: 12741814
Deposition date: 2003-03-10 Original release date: 2003-08-08
Authors: Chu, Josephine; Volk, David; Luxon, Bruce; Gorenstein, David; Hilser, Vincent
Citation: Ferreon, Josephine; Volk, David; Luxon, Bruce; Gorenstein, David; Hilser, Vincent. "Solution Structure, Dynamics, and Thermodynamics of the Native State Ensemble of the Sem-5 C-terminal SH3 Domain" Biochemistry 42, 5582-5591 (2003).
Assembly members:
SH3 Domain, polymer, 62 residues, Formula weight is not available
Natural source: Common Name: 6239 Taxonomy ID: C. elegans Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Caenorhabditis elegans
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
SH3 Domain: HMETKFVEALFDFNPQESGE
LAFKRGDVITLINKDDPNWW
EGQLNNRRGIFPSNYVAPYN
SN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 293 |
| 15N chemical shifts | 68 |
| 1H chemical shifts | 439 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SH3 subunit 1, monomer | 1 |
Entities:
Entity 1, SH3 subunit 1, monomer 62 residues - Formula weight is not available
| 1 | HIS | MET | GLU | THR | LYS | PHE | VAL | GLU | ALA | LEU | ||||
| 2 | PHE | ASP | PHE | ASN | PRO | GLN | GLU | SER | GLY | GLU | ||||
| 3 | LEU | ALA | PHE | LYS | ARG | GLY | ASP | VAL | ILE | THR | ||||
| 4 | LEU | ILE | ASN | LYS | ASP | ASP | PRO | ASN | TRP | TRP | ||||
| 5 | GLU | GLY | GLN | LEU | ASN | ASN | ARG | ARG | GLY | ILE | ||||
| 6 | PHE | PRO | SER | ASN | TYR | VAL | ALA | PRO | TYR | ASN | ||||
| 7 | SER | ASN |
Samples:
sample_1: SH3 Domain, [U-95% 13C; U-95% 15N], 2.0 mM
Ex-cond_1: pH: 4.8; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Varian UnityPlus 400 MHz
- Varian UnityPlus 750 MHz
Download simulated HSQC data in one of the following formats:
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