BMRB Entry 5767
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5767
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: 1H NMR structure determination of Viscotoxin C1 PubMed: 14580196
Deposition date: 2003-04-11 Original release date: 2003-12-18
Authors: Molinari, H.; Romagnoli, S.; Fogolari, F.; Catalano, M.; Urech, K.; Giannattasio, M.; Ragona, L.
Citation: Romagnoli, S.; Fogolari, F.; Catalano, M.; Zetta, L.; Schaller, G.; Urech, K.; Giannattasio, M.; Ragona, L.; Molinari, H.. "NMR solution structure of viscotoxin C1 from Viscum album species Coloratum ohwi: toward a structure-function analysis of viscotoxins" Biochemistry 42, 12503-12510 (2003).
Assembly members:
Cue2, polymer, 46 residues, Formula weight is not available
Natural source: Common Name: European mistletoe Taxonomy ID: 3972 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Viscum album
Experimental source: Production method: prufified from the natural source
Entity Sequences (FASTA):
Cue2: KSCCPNTTGRNIYNTCRFAG
GSRERCAKLSGCKIISASTC
PSDYPK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 278 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | protein Cue2 | 1 |
Entities:
Entity 1, protein Cue2 46 residues - Formula weight is not available
| 1 | LYS | SER | CYS | CYS | PRO | ASN | THR | THR | GLY | ARG | ||||
| 2 | ASN | ILE | TYR | ASN | THR | CYS | ARG | PHE | ALA | GLY | ||||
| 3 | GLY | SER | ARG | GLU | ARG | CYS | ALA | LYS | LEU | SER | ||||
| 4 | GLY | CYS | LYS | ILE | ILE | SER | ALA | SER | THR | CYS | ||||
| 5 | PRO | SER | ASP | TYR | PRO | LYS |
Samples:
sample_1: Cue2 0.7 mM; phosphate buffer 50 mM; H20 90%; D2O 10%
sample_2: Cue2 0.7 mM; phosphate buffer 50 mM; D2O 100%
sample_cond_1: ionic strength: 50 mM; pH: 3.6; pressure: 1 atm; temperature: 285 K
sample_cond_2: ionic strength: 50 mM; pH: 3.6; pressure: 1 atm; temperature: 295 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D TOCSY | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
| DQF-COSY | not available | not available | not available |
Software:
DYANA v1.4 - structure solution
DISCOVER v1997 version - refinement, structure solution
xwinnmr v2.6 - processing
XEASY v1.3 - data analysis
NMR spectrometers:
- Bruker DMX 500 MHz