BMRB Entry 5769
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5769
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Title: Solution structure of apo-CopAS46V from Bacillus subtilis PubMed: 12888353
Deposition date: 2003-04-11 Original release date: 2003-09-08
Authors: Banci, L.; Bertini, I.; Ciofi-Baffoni, S.; Gonnelli, L.; Su, X.
Citation: Banci, L.; Bertini, I.; Ciofi-Baffoni, S.; Gonnelli, L.; Su, X.. "A Core Mutation Affecting the Folding Properties of a Soluble Domain of the ATPase Protein CopA from Bacillus subtilis " J. Mol. Biol. 331, 473-484 (2003).
Assembly members:
CPx-type ATPase CopA, polymer, 76 residues, Formula weight is not available
Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Eubacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
CPx-type ATPase CopA: MLSEQKEIAMQVSGMTCAAC
AARIEKGLKRMPGVTDANVN
LATETVNVIYDPAETGTAAI
QEKIEKLGYHVVIEGR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 463 |
| 15N chemical shifts | 77 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CopA | 1 |
Entities:
Entity 1, CopA 76 residues - Formula weight is not available
| 1 | MET | LEU | SER | GLU | GLN | LYS | GLU | ILE | ALA | MET | ||||
| 2 | GLN | VAL | SER | GLY | MET | THR | CYS | ALA | ALA | CYS | ||||
| 3 | ALA | ALA | ARG | ILE | GLU | LYS | GLY | LEU | LYS | ARG | ||||
| 4 | MET | PRO | GLY | VAL | THR | ASP | ALA | ASN | VAL | ASN | ||||
| 5 | LEU | ALA | THR | GLU | THR | VAL | ASN | VAL | ILE | TYR | ||||
| 6 | ASP | PRO | ALA | GLU | THR | GLY | THR | ALA | ALA | ILE | ||||
| 7 | GLN | GLU | LYS | ILE | GLU | LYS | LEU | GLY | TYR | HIS | ||||
| 8 | VAL | VAL | ILE | GLU | GLY | ARG |
Samples:
sample_1: CPx-type ATPase CopA, [U-15N], 1.2 mM; phosphate 20 mM; DTT 2 mM; H2O 90%; D2O 10%
sample_cond_1: pH: 7.0; temperature: 298 K; ionic strength: 20 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
| HNHA | not available | not available | not available |
| HNHB | not available | not available | not available |
Software:
XWINNMR v2.6 - processing
Xeasy v1.3 - structure solution
DYANA v1.5 - structure solution
AMBER v5.0 - refinement
NMR spectrometers:
- Bruker AVANCE 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts