BMRB Entry 5867
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5867
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Title: Solution Structure of the third SH3 domain of human intersectin 2(KIAA1256)
Deposition date: 2003-07-14 Original release date: 2006-01-11
Authors: Suzuki, S.; Hatanaka, H.; Koshiba, S.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.
Citation: Suzuki, S.; Hatanaka, H.; Koshiba, S.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.. "Solution Structure of the third SH3 domain of human intersectin 2(KIAA1256)" . ., .-..
Assembly members:
INTERSECTIN 2, polymer, 69 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
INTERSECTIN 2: GSSGSSGGEEYIALYPYSSV
EPGDLTFTEGEEILVTQKDG
EWWTGSIGDRSGIFPSNYVK
PKDSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 289 |
| 15N chemical shifts | 67 |
| 1H chemical shifts | 392 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | INTERSECTIN 2 | 1 |
Entities:
Entity 1, INTERSECTIN 2 69 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLY | GLU | GLU | ||||
| 2 | TYR | ILE | ALA | LEU | TYR | PRO | TYR | SER | SER | VAL | ||||
| 3 | GLU | PRO | GLY | ASP | LEU | THR | PHE | THR | GLU | GLY | ||||
| 4 | GLU | GLU | ILE | LEU | VAL | THR | GLN | LYS | ASP | GLY | ||||
| 5 | GLU | TRP | TRP | THR | GLY | SER | ILE | GLY | ASP | ARG | ||||
| 6 | SER | GLY | ILE | PHE | PRO | SER | ASN | TYR | VAL | LYS | ||||
| 7 | PRO | LYS | ASP | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: INTERSECTIN 2, [U-13C; U-15N], 6.6 mM; phosphate buffer Na 20 mM; NaCl 100 mM; NaN3 0.02%; H2O 90%; D2O 10%
sample_cond_1: ionic strength: 0.12 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
Software:
xwinnmr v2.6 - collection
NMRPipe v20020425 - processing
NMRView v5.0.4 - data analysis
Kujira v0.771 - data analysis
Olivia v1.9.5 - data analysis
CYANA v1.0.7 - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB |
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