BMRB Entry 5913
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5913
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Title: NMR SOLUTION STRUCTURE OF CN12, A NOVEL ALPHA-SCORPION TOXIN FROM CENTRUROIDES NOXIUS, SHOWING THE IMPORTANCE OF SURFACE CHARGE DISTRIBUTION FOR NA+-CHANNEL AFINITY. PubMed: 15182366
Deposition date: 2003-08-20 Original release date: 2004-08-30
Authors: DEL RIO-PORTILLA, F.; POSSANI, L.; HERNANDEZ-MARIN, E.; WANKE, E.; PIMIENTA, G.; CORONAS, F.; ZAMUDIO, F.
Citation: DEL RIO-PORTILLA, F.; HERNANDEZ-MARIN, E.; PIMIENTA, G.; CORONAS, F.; ZAMUDIO, F.; Rodriguez de la Vega, R.; Wanke, E.; Possani, L.. "NMR solution structure of Cn12, a novel peptide from the Mexican scorpion Centruroides noxius with a typical beta-toxin sequence but with alpha-like physiological activity" Eur. J. Biochem. 271, 2504-2516 (2004).
Assembly members:
Neurotoxin Cn11, polymer, 67 residues, Formula weight is not available
Natural source: Common Name: Mexican scorpion Taxonomy ID: 6878 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Centruroides noxius
Experimental source: Production method: .
Entity Sequences (FASTA):
Neurotoxin Cn11: RDGYPLASNGCKFGCSGLGE
NNPTCNHVCEKKAGSDYGYC
YAWTCYCEHVAEGTVLWGDS
GTGPCRS
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| coupling constants | 22 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Neurotoxin Cn11 | 1 |
Entities:
Entity 1, Neurotoxin Cn11 67 residues - Formula weight is not available
| 1 | ARG | ASP | GLY | TYR | PRO | LEU | ALA | SER | ASN | GLY | ||||
| 2 | CYS | LYS | PHE | GLY | CYS | SER | GLY | LEU | GLY | GLU | ||||
| 3 | ASN | ASN | PRO | THR | CYS | ASN | HIS | VAL | CYS | GLU | ||||
| 4 | LYS | LYS | ALA | GLY | SER | ASP | TYR | GLY | TYR | CYS | ||||
| 5 | TYR | ALA | TRP | THR | CYS | TYR | CYS | GLU | HIS | VAL | ||||
| 6 | ALA | GLU | GLY | THR | VAL | LEU | TRP | GLY | ASP | SER | ||||
| 7 | GLY | THR | GLY | PRO | CYS | ARG | SER |
Samples:
sample_1: Neurotoxin Cn11 0.6 mM; H2O 90%; D2O 10%
sample_2: Neurotoxin Cn11 0.6 mM; D2O 100%
sample_cond_1: pH: 3.1; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF COSY | not available | not available | sample_cond_1 |
| 2D TOCSY | not available | not available | sample_cond_1 |
| AND 2D NOSEY | not available | not available | sample_cond_1 |
| DQF-COSY | not available | not available | sample_cond_1 |
| AND 2D NOESY | not available | not available | sample_cond_1 |
Software:
CNS v1.1 - refinement
NMRPipe v1 - processing
VNMR v6.1c - collection
NMR spectrometers:
- VARIAN UNITY PLUS 500 MHz