BMRB Entry 6028
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6028
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Title: Northeast Structural Genomics Consortium Target TT802 Ontario Center for Structural Proteomics Target Mth0803 assignment of ribosomal protein S17E
Deposition date: 2003-12-02 Original release date: 2004-05-15
Authors: Wu, Bin; Yee, Adelina; Ramelot, Theresa; Cort, John; Kennedy, Michael; Arrowsmith, Cheryl
Citation: Wu, Bin; Yee, Adelina; Ramelot, Theresa; Cort, John; Kennedy, Michael; Arrowsmith, Cheryl. "Solution structure of ribosomal protein S17E from Methanobacterium thermoautotrophicum" The BMRB entry is the only known published source for the data..
Assembly members:
ribosomal protein S17E, polymer, 62 residues, 7199 Da.
Natural source: Common Name: methanobacterium thermoautotrophicus Taxonomy ID: 145262 Superkingdom: Archaea Kingdom: not available Genus/species: Methanothermobacter thermoautotrophicum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ribosomal protein S17E: MGNIRTSFVKRIAKEMIETH
PGKFTDDFDTNKKLVEEFST
VSTKHLRNKIAGYITRIISQ
QK
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 436 |
| 13C chemical shifts | 264 |
| 15N chemical shifts | 64 |
| coupling constants | 49 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | mth0803 | 1 |
Entities:
Entity 1, mth0803 62 residues - 7199 Da.
| 1 | MET | GLY | ASN | ILE | ARG | THR | SER | PHE | VAL | LYS | ||||
| 2 | ARG | ILE | ALA | LYS | GLU | MET | ILE | GLU | THR | HIS | ||||
| 3 | PRO | GLY | LYS | PHE | THR | ASP | ASP | PHE | ASP | THR | ||||
| 4 | ASN | LYS | LYS | LEU | VAL | GLU | GLU | PHE | SER | THR | ||||
| 5 | VAL | SER | THR | LYS | HIS | LEU | ARG | ASN | LYS | ILE | ||||
| 6 | ALA | GLY | TYR | ILE | THR | ARG | ILE | ILE | SER | GLN | ||||
| 7 | GLN | LYS |
Samples:
sample_1: ribosomal protein S17E, [U-13C; U-15N], 1 mM; NaCL 450 mM
cond._set1: pH: 6.5 na; temperature: 298 K; ionic strength: 0.45 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N HSQC | not available | not available | not available |
| HNCO | not available | not available | not available |
| CBCACONH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCOCA | not available | not available | not available |
| CCTOCSY NH | not available | not available | not available |
| HCCTOCSY NH | not available | not available | not available |
| HNHA | not available | not available | not available |
| 13C HSQC | not available | not available | not available |
| HCCHCOSY | not available | not available | not available |
| HCCHTOCSY | not available | not available | not available |
| 15N NOESY | not available | not available | not available |
| 13C NOESY | not available | not available | not available |
| 13,13C 4D CCNOESY | not available | not available | not available |
Software:
SPARKY v3.95 - spectral analysis, assignemt
NMR spectrometers:
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts