BMRB Entry 6056
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6056
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Title: Yeast oligosaccharyltransferase subunit Ost4p PubMed: 15001703
Deposition date: 2003-12-24 Original release date: 2004-05-15
Authors: Zubkov, Sergey; Lennarz, William; Mohanty, Sergey
Citation: Zubkov, Sergey; Lennarz, William; Mohanty, Smita. "Structural basis for the function of a minimembrane protein subunit of yeast oligosaccharyltransferase" Proc. Natl. Acad. Sci. U. S. A. 101, 3821-3826 (2004).
Assembly members:
yeast oligosaccharyltransferase subunit ost4p, polymer, 36 residues, Formula weight is not available
Natural source: Common Name: Baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
yeast oligosaccharyltransferase subunit ost4p: MISDEQLNSLAITFGIVMMT
LIVIYHAVDSTMSPKN
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 226 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ost4p | 1 |
Entities:
Entity 1, ost4p 36 residues - Formula weight is not available
| 1 | MET | ILE | SER | ASP | GLU | GLN | LEU | ASN | SER | LEU | ||||
| 2 | ALA | ILE | THR | PHE | GLY | ILE | VAL | MET | MET | THR | ||||
| 3 | LEU | ILE | VAL | ILE | TYR | HIS | ALA | VAL | ASP | SER | ||||
| 4 | THR | MET | SER | PRO | LYS | ASN |
Samples:
sample_1: yeast oligosaccharyltransferase subunit ost4p 1.0 mM
sample_cond_1: pH*: 7.0; temperature: 298 K; pressure: 1 atm
sample_cond_2: pH*: 7.0; temperature: 311 K; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample_1 | not available | not available |
| 2D TOCSY | sample_1 | not available | not available |
| 2D DQF-COSY | sample_1 | not available | not available |
Software:
NMRPipe v2.2 - processing
NMRView v5.0.4 - data analysis
CYANA v1.0.6 - structure solution
ARIA v1.2 - refinement
NMR spectrometers:
- Bruker DMX 500 MHz
- Bruker DMX 750 MHz