BMRB Entry 6110
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6110
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: solution structure of a chimeric LEKTI-domain PubMed: 15366933
Deposition date: 2004-02-23 Original release date: 2004-09-28
Authors: Tidow, H.; Lauber, T.; Roesch, P.; Marx, U.
Citation: Tidow, H.; Lauber, T.; Vitzithum, K.; Sommerhoff, C.; Roesch, P.; Marx, U.. "Solution structure of a chimeric LEKTI domain reveals a chameleon sequence" Biochemistry 43, 11238-11247 (2004).
Assembly members:
mutant of serine proteinase inhibitor LEKTI, polymer, 55 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
mutant of serine proteinase inhibitor LEKTI: KNEDQEMCHEFQAFMKNGKL
FCPQDKKPIQSLDGIMFINK
CATCKMILEKEAKSQ
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 284 |
| 15N chemical shifts | 56 |
| coupling constants | 15 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Dom1PI | 1 |
Entities:
Entity 1, Dom1PI 55 residues - Formula weight is not available
| 1 | LYS | ASN | GLU | ASP | GLN | GLU | MET | CYS | HIS | GLU | ||||
| 2 | PHE | GLN | ALA | PHE | MET | LYS | ASN | GLY | LYS | LEU | ||||
| 3 | PHE | CYS | PRO | GLN | ASP | LYS | LYS | PRO | ILE | GLN | ||||
| 4 | SER | LEU | ASP | GLY | ILE | MET | PHE | ILE | ASN | LYS | ||||
| 5 | CYS | ALA | THR | CYS | LYS | MET | ILE | LEU | GLU | LYS | ||||
| 6 | GLU | ALA | LYS | SER | GLN |
Samples:
sample_1: mutant of serine proteinase inhibitor LEKTI 1.5 mM; potassium phosphate 10 mM
sample_2: mutant of serine proteinase inhibitor LEKTI, [U-100% 15N], 1.3 mM; potassium phosphate 10 mM
sample_cond_1: pH: 5.0; temperature: 298 K; ionic strength: 10 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D-TOCSY | not available | not available | sample_cond_1 |
| 2D-COSY | not available | not available | sample_cond_1 |
| 2D-NOESY | not available | not available | sample_cond_1 |
| 1H,15N-HSQC | not available | not available | sample_cond_1 |
| HNHA | not available | not available | sample_cond_1 |
| 3D-1H,15N-TOCSY-HSQC | not available | not available | sample_cond_1 |
| 3D-1H,15N-NOESY-HSQC | not available | not available | sample_cond_1 |
| 3D-1H,15N,15N-HMQC-NOESY-HSQC | not available | not available | sample_cond_1 |
Software:
NDEE -
NMRview -
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts