BMRB Entry 6135
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6135
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Title: Solution structure of conotoxin MrVIB PubMed: 15044438
Deposition date: 2004-03-03 Original release date: 2004-06-29
Authors: Daly, N.; Ekberg, J.; Thomas, L.; Adams, D.; Lewis, R.; Craik, D.
Citation: Daly, N.; Ekberg, J.; Thomas, L.; Adams, D.; Lewis, R.; Craik, D.. "Structures of mu O-conotoxins from Conus marmoreus: Inhibitors of TTX-sensitive and TTX-resistant sodium channels in mammalian sensory neurons" J. Biol. Chem. ., .-. (2004).
Assembly members:
MrVIB, polymer, 31 residues, 3404.8 Da.
Natural source: Common Name: cone snail Taxonomy ID: 42752 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Conus marmoreus
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
MrVIB: ACSKKWEYCIVPILGFVYCC
PGLICGPFVCV
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 174 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MrVIB | 1 |
Entities:
Entity 1, MrVIB 31 residues - 3404.8 Da.
| 1 | ALA | CYS | SER | LYS | LYS | TRP | GLU | TYR | CYS | ILE | ||||
| 2 | VAL | PRO | ILE | LEU | GLY | PHE | VAL | TYR | CYS | CYS | ||||
| 3 | PRO | GLY | LEU | ILE | CYS | GLY | PRO | PHE | VAL | CYS | ||||
| 4 | VAL |
Samples:
sample_1: MrVIB 1 mM; CD3CN 50%; H2O 40%; D2O 10%
sample_cond_1: pH: 3.5; pressure: 1 atm; temperature: 290 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample_1 | not available | sample_cond_1 |
| DQF-COSY | sample_1 | not available | sample_cond_1 |
| E-COSY | sample_1 | not available | sample_cond_1 |
Software:
XWINNMR v2.6 - collection, processing
XEASY v1.3.7 - data analysis
CNS v1.1 - refinement, structure solution
NMR spectrometers:
- Bruker DMX 750 MHz