BMRB Entry 6162
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6162
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Title: Full 1H, 13C and 15N Chemical Shift Assignments for the GII Domain of the yeast Linker Histone, Hho1p PubMed: 15050829
Deposition date: 2004-04-01 Original release date: 2005-02-07
Authors: Coles, Patrick; Thomas, Jean; Stott, Katherine; Stevens, Timothy; Ali, Tariq
Citation: Ali, Tariq; Coles, Patrick; Stevens, Timothy; Stott, Katherine; Thomas, Jean. "Two homologous domains of similar structure but different stability in the yeast linker histone, Hho1p" J. Mol. Biol. 338, 139-148 (2004).
Assembly members:
Yeast linker histone globular domain II, polymer, 88 residues, Formula weight is not available
Natural source: Common Name: Baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Yeast linker histone globular domain II: KASSPSSLTYKEMILKSMPQ
LNDGKGSSRIVLKKYVKDTF
SSKLKTSSNFDYLFNSAIKK
CVENGELVQPKGPSGIIKLN
KKKVKLST
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 299 |
| 15N chemical shifts | 89 |
| 1H chemical shifts | 620 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Hho1p GII | 1 |
Entities:
Entity 1, Hho1p GII 88 residues - Formula weight is not available
| 1 | LYS | ALA | SER | SER | PRO | SER | SER | LEU | THR | TYR | ||||
| 2 | LYS | GLU | MET | ILE | LEU | LYS | SER | MET | PRO | GLN | ||||
| 3 | LEU | ASN | ASP | GLY | LYS | GLY | SER | SER | ARG | ILE | ||||
| 4 | VAL | LEU | LYS | LYS | TYR | VAL | LYS | ASP | THR | PHE | ||||
| 5 | SER | SER | LYS | LEU | LYS | THR | SER | SER | ASN | PHE | ||||
| 6 | ASP | TYR | LEU | PHE | ASN | SER | ALA | ILE | LYS | LYS | ||||
| 7 | CYS | VAL | GLU | ASN | GLY | GLU | LEU | VAL | GLN | PRO | ||||
| 8 | LYS | GLY | PRO | SER | GLY | ILE | ILE | LYS | LEU | ASN | ||||
| 9 | LYS | LYS | LYS | VAL | LYS | LEU | SER | THR |
Samples:
sample_1: Yeast linker histone globular domain II, [U-95% 13C; U-95% 15N], 1.0 mM; sodium phosphate 250 mM; EDTA 1 mM; DTT 1 mM; DSS 10 mM; D2O 99.8%
Ex-cond_1: ionic strength: 0.250 M; pH: 7.0; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY-15N-HSQC | sample_1 | not available | Ex-cond_1 |
| 15N-HSQC-TOCSY | sample_1 | not available | Ex-cond_1 |
| HNCA | sample_1 | not available | Ex-cond_1 |
| HNCACB | sample_1 | not available | Ex-cond_1 |
| HN(CO)CA | sample_1 | not available | Ex-cond_1 |
| CBCA(CO)NH | sample_1 | not available | Ex-cond_1 |
| (H)CC(CO)NH-TOCSY | sample_1 | not available | Ex-cond_1 |
| HC(C)H-TOCSY | sample_1 | not available | Ex-cond_1 |
| NOESY-13C-HSQC | sample_1 | not available | Ex-cond_1 |
Software:
AZARA v2.7 - Spectrum processing
ANSIG v3.3 - Assignment
NMR spectrometers:
- Bruker DRX 500 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts