BMRB Entry 6180
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6180
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Title: Solution structure of the 15th domain of LEKTI PubMed: 15366933
Deposition date: 2004-04-15 Original release date: 2005-05-17
Authors: Vitzithum, Klaus; Roesch, Paul; Marx, Ute
Citation: Tidow, H.; Lauber, T.; Vitzithum, Klaus; Sommerhoff, C.; Roesch, Paul; Marx, Ute. "The Solution structure of a chimeric LEKTI domain reveals a chameleon sequence" Biochemistry 43, 11238-11247 (2004).
Assembly members:
LEKTI Domain 15, polymer, 78 residues, 8569.6 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
LEKTI Domain 15: GPDSEMCKDYRVLPRIGYLC
PKDLKPVCGDDGQTYNNPCM
LCHENLIRQTNTHIRSTGKC
EESSTPGTTAASMPPSDE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 423 |
| 15N chemical shifts | 79 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | LEKTI Domain 15 | 1 |
Entities:
Entity 1, LEKTI Domain 15 78 residues - 8569.6 Da.
| 1 | GLY | PRO | ASP | SER | GLU | MET | CYS | LYS | ASP | TYR | ||||
| 2 | ARG | VAL | LEU | PRO | ARG | ILE | GLY | TYR | LEU | CYS | ||||
| 3 | PRO | LYS | ASP | LEU | LYS | PRO | VAL | CYS | GLY | ASP | ||||
| 4 | ASP | GLY | GLN | THR | TYR | ASN | ASN | PRO | CYS | MET | ||||
| 5 | LEU | CYS | HIS | GLU | ASN | LEU | ILE | ARG | GLN | THR | ||||
| 6 | ASN | THR | HIS | ILE | ARG | SER | THR | GLY | LYS | CYS | ||||
| 7 | GLU | GLU | SER | SER | THR | PRO | GLY | THR | THR | ALA | ||||
| 8 | ALA | SER | MET | PRO | PRO | SER | ASP | GLU |
Samples:
sample_1: LEKTI Domain 15, [U-15N], 1.6 mM
sample_2: LEKTI Domain 15 2.3 mM
sample_cond_1: pH: 6.4; temperature: 298 K; ionic strength: 50 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D-NOESY | not available | not available | sample_cond_1 |
| 2D-TOCSY | not available | not available | sample_cond_1 |
| 2D-COESY | not available | not available | sample_cond_1 |
| 1H,15N-HSQC | not available | not available | sample_cond_1 |
| HNHA | not available | not available | sample_cond_1 |
| 3D-1H,1H,15-NOESY-HSQC | not available | not available | sample_cond_1 |
| 3D-1H,1H,15N-TOCSY-HSQC | not available | not available | sample_cond_1 |
| 3D-1H,15N,15N-HMQC-NOESY-HSQC | not available | not available | sample_cond_1 |
Software:
NMRview v5.0.4 -
NDEE, Spinup Inc. -
NMR spectrometers:
- Bruker DRX 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts