BMRB Entry 6427
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6427
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Title: Solution structure of a recombinant type I sculpin antifreeze protein PubMed: 15697223
Deposition date: 2004-12-10 Original release date: 2005-06-02
Authors: Kwan, A.; Fairley, K.; Anderberg, P.; Liew, C.; Harding, M.; Mackay, J.
Citation: Kwan, A.; Fairley, K.; Anderberg, P.; Liew, C.; Harding, M.; Mackay, J.. "Solution structure of a recombinant type I sculpin antifreeze protein." Biochemistry 44, 1980-1988 (2005).
Assembly members:
SS3, polymer, 35 residues, 3083.5 Da.
Natural source: Common Name: Shorthorn sculpin Taxonomy ID: 8097 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Myoxocephalus scorpius
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
SS3: GSMNAPARAAAKTAADALAA
AKKTAADAAAAAAAA
- assigned_chemical_shifts
- RDCs
| Data type | Count |
| 13C chemical shifts | 204 |
| 15N chemical shifts | 66 |
| 1H chemical shifts | 390 |
| residual dipolar couplings | 18 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | rSS3 | 1 |
Entities:
Entity 1, rSS3 35 residues - 3083.5 Da.
| 1 | GLY | SER | MET | ASN | ALA | PRO | ALA | ARG | ALA | ALA | ||||
| 2 | ALA | LYS | THR | ALA | ALA | ASP | ALA | LEU | ALA | ALA | ||||
| 3 | ALA | LYS | LYS | THR | ALA | ALA | ASP | ALA | ALA | ALA | ||||
| 4 | ALA | ALA | ALA | ALA | ALA |
Samples:
sample_1: SS30.8 – 1.2 mM; H2O 95%; D2O 5%
sample_2: SS3, [U-15N; U-13C], 0.8 – 1.2 mM; H2O 95%; D2O 5%
sample_3: SS3, [U-15N], 0.1 – 0.3 mM
sample_cond_1: ionic strength: . mM; pH: 5.0; pressure: 1 atm; temperature: 268 K
sample_cond_2: ionic strength: . mM; pH: 5.0; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D TOCSY | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
| DQF-COSY | not available | not available | not available |
| HNHA | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCA-ECOSY | not available | not available | not available |
| HET-NOE | not available | not available | not available |
| 15N-HSQC | not available | not available | not available |
| 13C-HSQC | not available | not available | not available |
Software:
xwinnmr v2.5 - processing
XEASY v1.3.13 - data analysis
SPARKY v3 - data analysis
ARIA v1.2 - structure solution, refinement
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
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