BMRB Entry 6493
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6493
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Title: Assignment of the 1H, 13C, and 15N resonances of URNdesign, a computationally redisgned RRM protein
Deposition date: 2005-02-07 Original release date: 2005-11-03
Authors: Dobson, Neil; Dantas, Gautam; Varani, Gabriele
Citation: Dobson, Neil; Dantas, Gautam; Varani, Gabriele. "Letter to the Editor: 1H, 13C and 15N resonance assignments of URNdesign, a computationally redesigned RRM protein" J. Biomol. NMR ., .-..
Assembly members:
URNdesign, polymer, 107 residues, 11453 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
URNdesign: MDSPDLGSTPPHTEPSQVVL
ITNINPEVPKEKLQALLYAL
ASSQGDILDIVVDLSDDNSG
KAYIVFATQESAQAFVEAFQ
GYPFQGNPLVITFSETPQSQ
VAEDGSL
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 717 |
| 13C chemical shifts | 443 |
| 15N chemical shifts | 104 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | URNdesign | 1 |
Entities:
Entity 1, URNdesign 107 residues - 11453 Da.
| 1 | MET | ASP | SER | PRO | ASP | LEU | GLY | SER | THR | PRO | ||||
| 2 | PRO | HIS | THR | GLU | PRO | SER | GLN | VAL | VAL | LEU | ||||
| 3 | ILE | THR | ASN | ILE | ASN | PRO | GLU | VAL | PRO | LYS | ||||
| 4 | GLU | LYS | LEU | GLN | ALA | LEU | LEU | TYR | ALA | LEU | ||||
| 5 | ALA | SER | SER | GLN | GLY | ASP | ILE | LEU | ASP | ILE | ||||
| 6 | VAL | VAL | ASP | LEU | SER | ASP | ASP | ASN | SER | GLY | ||||
| 7 | LYS | ALA | TYR | ILE | VAL | PHE | ALA | THR | GLN | GLU | ||||
| 8 | SER | ALA | GLN | ALA | PHE | VAL | GLU | ALA | PHE | GLN | ||||
| 9 | GLY | TYR | PRO | PHE | GLN | GLY | ASN | PRO | LEU | VAL | ||||
| 10 | ILE | THR | PHE | SER | GLU | THR | PRO | GLN | SER | GLN | ||||
| 11 | VAL | ALA | GLU | ASP | GLY | SER | LEU |
Samples:
sample_1: URNdesign 1.0 mM
conditions_1: pH: 6.8; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N-HSQC | sample_1 | isotropic | conditions_1 |
| HNCO | sample_1 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
| HBHA(CO)NH | sample_1 | not available | conditions_1 |
| HN(CO)CA | sample_1 | not available | conditions_1 |
| 3D_15N_TOCSY | sample_1 | not available | conditions_1 |
| HCCH_TOCSY | sample_1 | not available | conditions_1 |
| 3D_15N_NOESY | sample_1 | not available | conditions_1 |
| 3D_13C_NOESY | sample_1 | not available | conditions_1 |
| 2D_D2O_TOCSY | sample_1 | not available | conditions_1 |
| 2D_D2O_NOESY | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 500MHz MHz
- Bruker Avance 750MHz MHz
- Varian . 600MHz MHz
Related Database Links:
| PDB |
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