BMRB Entry 6682
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR6682
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Title: Solution Structure of a Human C2H2-type Zinc Finger Protein PubMed: 18287285
Deposition date: 2005-06-09 Original release date: 2005-07-27
Authors: Lytle, B.; Peterson, F.; Volkman, B.
Citation: Hayes, P.; Lytle, B.; Volkman, B.; Peterson, F.. "The solution structure of ZNF593 from Homo sapiens reveals a zinc finger in a predominantly unstructured protein" Protein Sci. 17, 571-576 (2008).
Assembly members:
Zinc finger protein 593, polymer, 124 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
Zinc finger protein 593: GHHHHHHLEKAKRRRPDLDE
IHRELRPQGSARPQPDPNAE
FDPDLPGGGLHRCLACARYF
IDSTNLKTHFRSKDHKKRLK
QLSVEPYSQEEAERAAGMGS
YVPPRRLAVPTEVSTEVPEM
DTST
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 404 |
| 13C chemical shifts | 278 |
| 15N chemical shifts | 68 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Zinc finger protein 593 | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, Zinc finger protein 593 124 residues - Formula weight is not available
| 1 | GLY | HIS | HIS | HIS | HIS | HIS | HIS | LEU | GLU | LYS | ||||
| 2 | ALA | LYS | ARG | ARG | ARG | PRO | ASP | LEU | ASP | GLU | ||||
| 3 | ILE | HIS | ARG | GLU | LEU | ARG | PRO | GLN | GLY | SER | ||||
| 4 | ALA | ARG | PRO | GLN | PRO | ASP | PRO | ASN | ALA | GLU | ||||
| 5 | PHE | ASP | PRO | ASP | LEU | PRO | GLY | GLY | GLY | LEU | ||||
| 6 | HIS | ARG | CYS | LEU | ALA | CYS | ALA | ARG | TYR | PHE | ||||
| 7 | ILE | ASP | SER | THR | ASN | LEU | LYS | THR | HIS | PHE | ||||
| 8 | ARG | SER | LYS | ASP | HIS | LYS | LYS | ARG | LEU | LYS | ||||
| 9 | GLN | LEU | SER | VAL | GLU | PRO | TYR | SER | GLN | GLU | ||||
| 10 | GLU | ALA | GLU | ARG | ALA | ALA | GLY | MET | GLY | SER | ||||
| 11 | TYR | VAL | PRO | PRO | ARG | ARG | LEU | ALA | VAL | PRO | ||||
| 12 | THR | GLU | VAL | SER | THR | GLU | VAL | PRO | GLU | MET | ||||
| 13 | ASP | THR | SER | THR |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: Zinc finger protein 593, [U-15N; U-13C], 0.6 mM; Bis Tris 10 mM; NaCl 100 mM; DTT 2 mM; H2O 90%; D2O 10%
sample_cond_1: pH: 6.0; temperature: 298 K; ionic strength: 100 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | sample_1 | not available | sample_cond_1 |
| HNCA | sample_1 | not available | sample_cond_1 |
| HNCO | sample_1 | not available | sample_cond_1 |
| HN(CO)CA | sample_1 | not available | sample_cond_1 |
| HNCACB | sample_1 | not available | sample_cond_1 |
| HN(CA)CO | sample_1 | not available | sample_cond_1 |
| C(CO)NH | sample_1 | not available | sample_cond_1 |
| HBHA(CO)NH | sample_1 | not available | sample_cond_1 |
| HCCH-TOCSY | sample_1 | not available | sample_cond_1 |
| 3D 13C-separated NOESY (aliph) | sample_1 | not available | sample_cond_1 |
| 3D 15N-separated NOESY | sample_1 | not available | sample_cond_1 |
| 3D 13C-separated NOESY (AROMATIC) | sample_1 | not available | sample_cond_1 |
Software:
XWINNMR v3.5 - collection
NMRPipe v2004 - processing
SPSCAN v1.1.0 - data analysis
XEASY v1.3 - data analysis
GARANT v2.1 - data analysis
CYANA v1.0.6 - structure solution
XPLOR-NIH v2.0.6 - refinement
NMR spectrometers:
- Bruker AVANCE 600 MHz
Related Database Links:
| SWISS-PROT | O00488 |
| GenBank | ABZ92327 AAH19267 AAH02580 |
| DBJ | BAG34867 BAA20369 |
| PDB |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
or all simulated shifts