BMRB Entry 6749
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6749
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Title: Solution structure of the NRSF/REST-mSin3B PAH1 complex
Deposition date: 2005-07-26 Original release date: 2005-12-13
Authors: Nishimura, Yoshifumi; Nomura, Mitsuru
Citation: Nomura, Mitsuru; Uda-Tochio, Hiroko; Murai, Kiyohito; Mori, Nozomu; Nishimura, Yoshifumi. "The Neural Repressor NRSF/REST Binds the PAH1 Domain of the Sin3 Corepressor by Using its Distinct Short Hydrophobic Helix." J. Mol. Biol. 354, 903-915 (2005).
Assembly members:
mSin3B PAH1, polymer, 80 residues, Formula weight is not available
NRSF/REST, polymer, 15 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
mSin3B PAH1: EKLPVHVEDALTYLDQVKIR
FGSDPATYNGFLEIMKEFKS
QSIDTPGVIRRVSQLFHEHP
DLIVGFNAFLPLGYRIDIPK
NRSF/REST: APQLIMLANVALTGE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 364 |
| 15N chemical shifts | 78 |
| 1H chemical shifts | 642 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | mSin3B PAH1 | 1 |
| 2 | NRSF/REST | 2 |
Entities:
Entity 1, mSin3B PAH1 80 residues - Formula weight is not available
| 1 | GLU | LYS | LEU | PRO | VAL | HIS | VAL | GLU | ASP | ALA | |
| 2 | LEU | THR | TYR | LEU | ASP | GLN | VAL | LYS | ILE | ARG | |
| 3 | PHE | GLY | SER | ASP | PRO | ALA | THR | TYR | ASN | GLY | |
| 4 | PHE | LEU | GLU | ILE | MET | LYS | GLU | PHE | LYS | SER | |
| 5 | GLN | SER | ILE | ASP | THR | PRO | GLY | VAL | ILE | ARG | |
| 6 | ARG | VAL | SER | GLN | LEU | PHE | HIS | GLU | HIS | PRO | |
| 7 | ASP | LEU | ILE | VAL | GLY | PHE | ASN | ALA | PHE | LEU | |
| 8 | PRO | LEU | GLY | TYR | ARG | ILE | ASP | ILE | PRO | LYS |
Entity 2, NRSF/REST 15 residues - Formula weight is not available
| 1 | ALA | PRO | GLN | LEU | ILE | MET | LEU | ALA | ASN | VAL | ||||
| 2 | ALA | LEU | THR | GLY | GLU |
Samples:
sample_1: mSin3B PAH1, [U-13C; U-15N], 0.8 mM; NRSF/REST 0.8 mM; potassium phosphate buffer 20 mM
condition_1: pH: 7.5; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| HNCO | not available | not available | not available |
| HN(CA)CO | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HBHA(CO)NH | not available | not available | not available |
| HN(CA)HA | not available | not available | not available |
| 1H-13C HSQC | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| CCH-TOCSY | not available | not available | not available |
| 13C/15N-FILTERED-NOESY | not available | not available | not available |
| 13C/15N-FILTERED-TOCSY | not available | not available | not available |
Software:
NMRPipe v2004 -
NMRView v5.0.4 -
NMR spectrometers:
- Bruker Avance 700 MHz
Related Database Links:
| PDB | |
| DBJ | BAB82460 BAD92987 BAE33741 BAG10902 |
| GB | AAA98503 AAB17211 AAB94893 AAB94894 AAC50114 |
| PRF | 2106430A |
| REF | NP_001095350 NP_001180437 NP_005603 NP_035393 NP_113976 |
| SP | O54963 Q13127 Q8VIG1 |
| TPG | DAA28644 DAA28661 |
Download simulated HSQC data in one of the following formats:
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