BMRB Entry 6751
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6751
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Title: Solution Structure of Asl1650, an Acyl Carrier Protein from Anabaena sp. PCC 7120 with a Variant Phosphopantetheinylation-Site Sequence PubMed: 16597827
Deposition date: 2005-07-26 Original release date: 2005-08-22
Authors: Johnson, Margaret; Peti, Wolfgang; Herrmann, Torsten; Wilson, Ian; Wuthrich, Kurt
Citation: Johnson, Margaret; Peti, Wolfgang; Herrmann, Torsten; Wilson, Ian; Wuthrich, Kurt. "Solution structure of Asl1650, an acyl carrier protein from Anabaena sp. PCC 7120 with a variant phosphopantetheinylation-site sequence" Protein Sci. 15, 1030-1041 (2006).
Assembly members:
Asl1650, polymer, 88 residues, 10080.4 Da.
Natural source: Common Name: Anabaena sp. PCC 7120 Taxonomy ID: 103690 Superkingdom: Eubacteria Kingdom: not available Genus/species: Anabaena sp. PCC 7120
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Asl1650: GSHMKTIQPASVEDIQSWLI
DQFAQQLDVDPDDIDMEESF
DNYDLNSSKALILLGRLEKW
LGKELNPVLIFNYPTIAQLA
KRLGELYL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 360 |
| 15N chemical shifts | 93 |
| 1H chemical shifts | 647 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Asl1650 | 1 |
Entities:
Entity 1, Asl1650 88 residues - 10080.4 Da.
| 1 | GLY | SER | HIS | MET | LYS | THR | ILE | GLN | PRO | ALA | ||||
| 2 | SER | VAL | GLU | ASP | ILE | GLN | SER | TRP | LEU | ILE | ||||
| 3 | ASP | GLN | PHE | ALA | GLN | GLN | LEU | ASP | VAL | ASP | ||||
| 4 | PRO | ASP | ASP | ILE | ASP | MET | GLU | GLU | SER | PHE | ||||
| 5 | ASP | ASN | TYR | ASP | LEU | ASN | SER | SER | LYS | ALA | ||||
| 6 | LEU | ILE | LEU | LEU | GLY | ARG | LEU | GLU | LYS | TRP | ||||
| 7 | LEU | GLY | LYS | GLU | LEU | ASN | PRO | VAL | LEU | ILE | ||||
| 8 | PHE | ASN | TYR | PRO | THR | ILE | ALA | GLN | LEU | ALA | ||||
| 9 | LYS | ARG | LEU | GLY | GLU | LEU | TYR | LEU |
Samples:
sample_1: Asl1650, [U-15N; U-13C], 2 mM; phosphate buffer 20 mM; NaCl 250 mM; NaN3 2 mM
sample_2: Asl1650, [U-15N], 2 mM; phosphate buffer 20 mM; NaCl 250 mM; NaN3 2 mM
sample_3: Asl1650 2 mM; phosphate buffer 20 mM; NaCl 250 mM; NaN3 2 mM
conditions_1: ionic strength: 0.25 M; pH: 6.0; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNCO | not available | not available | not available |
| HBHA(CO)NH | not available | not available | not available |
| 15N-resolved [1H,1H]-TOCSY | not available | not available | not available |
| HC(CCO)NH | not available | not available | not available |
| HC(C)H-TOCSY | not available | not available | not available |
| 2D [1H,1H]-COSY | not available | not available | not available |
| 2D [1H,1H]-TOCSY | not available | not available | not available |
| 2D [1H,1H]-NOESY | not available | not available | not available |
| 3D 15N-resolved [1H,1H]-NOESY | not available | not available | not available |
| 3D 13C-resolved [1H,1H]-NOESY | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 900 MHz
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