BMRB Entry 6844
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: LACS
BMRB Entry DOI: doi:10.13018/BMR6844
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Title: Solution Structure of Drosophila melanogaster SNF RBD2 PubMed: 19727396
Deposition date: 2005-09-29 Original release date: 2009-10-20
Authors: Cui, G.; Li, C.; Jin, C.; Xia, B.
Citation: Jicheng, Hu; Gaofeng, Cui; Congmin, Li; Cong, Liu; Erchang, Shang; Luhua, Lai; Changwen, Jin; Jiwu, Wang; Bin, Xia. "Structure and Novel Functional Mechanism of Drosophila SNF in Sex-Lethal Splicing" PLoS One 4, 6890-. (2009).
Assembly members:
U1 small nuclear ribonucleoprotein A, polymer, 83 residues, Formula weight is not available
Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
U1 small nuclear ribonucleoprotein A: AQTEQPPNQILFLTNLPEET
NEMMLSMLFNQFPGFKEVRL
VPNRHDIAFVEFTTELQSNA
AKEALQGFKITPTHAMKITF
AKK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 339 |
| 15N chemical shifts | 90 |
| 1H chemical shifts | 601 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | U1 small nuclear ribonucleoprotein A | 1 |
Entities:
Entity 1, U1 small nuclear ribonucleoprotein A 83 residues - Formula weight is not available
| 1 | ALA | GLN | THR | GLU | GLN | PRO | PRO | ASN | GLN | ILE | ||||
| 2 | LEU | PHE | LEU | THR | ASN | LEU | PRO | GLU | GLU | THR | ||||
| 3 | ASN | GLU | MET | MET | LEU | SER | MET | LEU | PHE | ASN | ||||
| 4 | GLN | PHE | PRO | GLY | PHE | LYS | GLU | VAL | ARG | LEU | ||||
| 5 | VAL | PRO | ASN | ARG | HIS | ASP | ILE | ALA | PHE | VAL | ||||
| 6 | GLU | PHE | THR | THR | GLU | LEU | GLN | SER | ASN | ALA | ||||
| 7 | ALA | LYS | GLU | ALA | LEU | GLN | GLY | PHE | LYS | ILE | ||||
| 8 | THR | PRO | THR | HIS | ALA | MET | LYS | ILE | THR | PHE | ||||
| 9 | ALA | LYS | LYS |
Samples:
sample_1: U1 small nuclear ribonucleoprotein A, [U-15N; U-13C], 1 mM; phosphate buffer 50 mM; EDTA 1 mM; NaCl 50 mM; H2O 90%; D2O 10%
sample_cond_1: ionic strength: 250 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
Software:
xwinnmr v3.5 - collection
NMRPipe v2.1 - processing
NMRView v5 - data analysis
CYANA v1.0.6 - structure solution
AMBER v7.0 - refinement
NMR spectrometers:
- Bruker AVANCE 600 MHz
Related Database Links:
| PDB | |
| GB | AAA28441 AAA28903 AAF46017 AAL29039 ACL84772 |
| REF | NP_511045 XP_001355411 XP_001964014 XP_001976948 XP_001992159 |
| SP | P43332 |
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