BMRB Entry 6872
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6872
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Title: Solution structure of kalata B8 PubMed: 16207177
Deposition date: 2005-10-20 Original release date: 2008-09-24
Authors: Daly, N.; Clark, R.; Plan, M.; Craik, D.
Citation: Daly, N.; Clark, R.; Plan, M.; Craik, D.. "Kalata B8, a novel antiviral circular protein, exhibits conformational flexibility in the cystine knot motif" Biochem. J. 393, 619-626 (2006).
Assembly members:
kalata B8, polymer, 31 residues, Formula weight is not available
Natural source: Common Name: Oldenlandia affinis Taxonomy ID: 60225 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Oldenlandia affinis
Experimental source: Production method: recombinate technology
Entity Sequences (FASTA):
kalata B8: CGETCLLGTCYTTGCTCNKY
RVCTKDGSVLN
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 183 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | kalata B8 | 1 |
Entities:
Entity 1, kalata B8 31 residues - Formula weight is not available
| 1 | CYS | GLY | GLU | THR | CYS | LEU | LEU | GLY | THR | CYS | ||||
| 2 | TYR | THR | THR | GLY | CYS | THR | CYS | ASN | LYS | TYR | ||||
| 3 | ARG | VAL | CYS | THR | LYS | ASP | GLY | SER | VAL | LEU | ||||
| 4 | ASN |
Samples:
sample_1: kalata B8 1 mM; H2O 90%; D2O 10%
sample_cond_1: pH: 3.5; pressure: 1 atm; temperature: 290 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
| DQF-COSY | not available | not available | not available |
| E-COSY | not available | not available | not available |
Software:
xwinnmr v3.5 - collection
XEASY v1.3.7 - data analysis
DYANA v1.5 - refinement
CNS v1.1 - structure solution
NMR spectrometers:
- Bruker AVANCE 500 MHz