BMRB Entry 7036
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7036
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for 3rd SH3 domain of human NCK2 adaptor protein PubMed: 16752908
Deposition date: 2006-03-29 Original release date: 2011-08-11
Authors: Liu, Jingxian
Citation: Liu, Jingxian; Li, Minfen; Ran, Xiaoyuan; Fan, Jing-song; Song, Jianxing. "Structural Insight into the Binding Diversity between the Human Nck2 SH3 Domains and Proline-Rich Proteins(,)" Biochemistry 45, 7171-7184 (2006).
Assembly members:
NCK2 3rd SH3, polymer, 61 residues, 6822 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
NCK2 3rd SH3: GSHVVQTLYPFSSVTEEELN
FEKGETMEVIEKPENDPEWW
KCKNARGQVGLVPKNYVVVL
S
- assigned_chemical_shifts
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
| Data type | Count |
| 13C chemical shifts | 177 |
| 15N chemical shifts | 55 |
| 1H chemical shifts | 338 |
| T1 relaxation values | 54 |
| T2 relaxation values | 54 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NCK2 3rd SH3 | 1 |
Entities:
Entity 1, NCK2 3rd SH3 61 residues - 6822 Da.
| 1 | GLY | SER | HIS | VAL | VAL | GLN | THR | LEU | TYR | PRO | ||||
| 2 | PHE | SER | SER | VAL | THR | GLU | GLU | GLU | LEU | ASN | ||||
| 3 | PHE | GLU | LYS | GLY | GLU | THR | MET | GLU | VAL | ILE | ||||
| 4 | GLU | LYS | PRO | GLU | ASN | ASP | PRO | GLU | TRP | TRP | ||||
| 5 | LYS | CYS | LYS | ASN | ALA | ARG | GLY | GLN | VAL | GLY | ||||
| 6 | LEU | VAL | PRO | LYS | ASN | TYR | VAL | VAL | VAL | LEU | ||||
| 7 | SER |
Samples:
sample_1: NCK2 3rd SH3, [U-95% 15N], 2.0 mM; phosphate 20 mM; DTT 10 mM; D2O 10%
sample_2: NCK2 3rd SH3, [U-95% 13C; U-95% 15N], 2.0 mM; phosphate 20 mM; DTT 10 mM; D2O 10%
sample_3: NCK2 3rd SH3, [U-95% 13C; U-95% 15N], 2.0 mM; phosphate 20 mM; DTT 10 mM; D2O 70%
conditions_1: pH: 6.8; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N HSQC | sample_1 | not available | not available |
| HSQC TOCSY | sample_1 | not available | not available |
| HSQC NOESY | sample_1 | not available | not available |
| HNCACB/CBCANH | sample_2 | not available | not available |
| HCCH TOCSY/HCCH NOESY | sample_3 | not available | not available |
| T1/T2/HetNOE | sample_1 | not available | not available |
Software:
NMRPipe - raw data processing
NMRView - peak assignment
NMR spectrometers:
- Bruker Avance 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts