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Biological Magnetic Resonance Data Bank


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BMRB Entry 7058

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7058
MolProbity Validation Chart

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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for calponin homology domain of human MICAL_1   PubMed: 17043746

Deposition date: 2006-04-08 Original release date: 2006-06-27

Authors: Dai, Haiming; Sun, Hongbin; Zhang, Jiahai; Xiong, shangmin; Xu, Jian; Wu, Jihui; Shi, yunyu

Citation: Sun, Hongbin; Dai, Haiming; Zhang, Jiahai; Jin, Xianju; Xiong, Shangmin; Xu, Jian; Wu, Jihui; Shi, Yunyu. "Solution structure of calponin homology domain of Human MICAL-1"  J. Biomol. NMR 36, 295-300 (2006).

Assembly members:
MICAL_1 CH, polymer, 118 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
MICAL_1 CH: MGHHHHHHMGSAGTQEELLR WCQEQTAGYPGVHVSDLSSS WADGLALCALVYRLQPGLLE PSELQGLGALEATAWALKVA ENELGITPVVSAQAVVAGSD PLGLIAYLSHFHSAFKSM

Data sets:
Data typeCount
13C chemical shifts426
15N chemical shifts115
1H chemical shifts736

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MICAL_1 CH1

Entities:

Entity 1, MICAL_1 CH 118 residues - Formula weight is not available

1   METGLYHISHISHISHISHISHISMETGLY
2   SERALAGLYTHRGLNGLUGLULEULEUARG
3   TRPCYSGLNGLUGLNTHRALAGLYTYRPRO
4   GLYVALHISVALSERASPLEUSERSERSER
5   TRPALAASPGLYLEUALALEUCYSALALEU
6   VALTYRARGLEUGLNPROGLYLEULEUGLU
7   PROSERGLULEUGLNGLYLEUGLYALALEU
8   GLUALATHRALATRPALALEULYSVALALA
9   GLUASNGLULEUGLYILETHRPROVALVAL
10   SERALAGLNALAVALVALALAGLYSERASP
11   PROLEUGLYLEUILEALATYRLEUSERHIS
12   PHEHISSERALAPHELYSSERMET

Samples:

sample_1: MICAL_1 CH, [U-15N], 1.5 ± 0.1 mM; phosphate buffer 50 ± 1 mM; NaCl 50 ± 1 mM; D2O 10 ± 1 %

sample_2: MICAL_1 CH, [U-13C; U-15N], 1.5 ± 0.1 mM; phosphate buffer 50 ± 1 mM; phosphate buffer 50 ± 1 mM; D2O 10 ± 1 %

sample_3: MICAL_1 CH, [U-13C; U-15N], 1.5 ± 0.1 mM; phosphate buffer 50 ± 0.1 mM; NaCl 50 ± 0.1 mM; D2O 99.96 ± 1 %

conditions_1: pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCnot availablenot availableconditions_1
CBCACONHnot availablenot availableconditions_1
CBCANHnot availablenot availableconditions_1
HNCOnot availablenot availableconditions_1
HBHACBCACONHnot availablenot availableconditions_1
HCCONH-tocsynot availablenot availableconditions_1
CCONH-tocsynot availablenot availableconditions_1
15N_NOESYnot availablenot availableconditions_1
13C_NOESYnot availablenot availableconditions_1
HNCACOnot availablenot availableconditions_1
HCCH-tocsynot availablenot availableconditions_1
HCCH-cosynot availablenot availableconditions_1

Software:

NMRPipe v2.3 - Data analysis

SPARKY v3 -

CNS v1.1 -

Molmol v2k.2 -

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DMX 600 MHz

Related Database Links:

BMRB 11085
PDB
DBJ BAB13949 BAB15124 BAB86289 BAD18727 BAH12301
EMBL CAB59266
GB AAH09972 AAH42144 AAH52983 AIC63549 EAW48341
REF NP_001152763 NP_001273542 NP_073602 XP_001090339 XP_001153132
SP Q8TDZ2

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts