BMRB Entry 7099
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7099
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Title: NMR Solution Structure of VP9 from White Spot Syndrome Virus PubMed: 16956937
Deposition date: 2006-05-05 Original release date: 2008-07-16
Authors: Liu, Y.; Song, J.; Hew, C.
Citation: Liu, Y.; Wu, J.; Song, J.; Sivaraman, J.; Hew, C.. "Identification of a Novel Nonstructural Protein VP9 from White Spot Syndrome Virus: Its Structure Reveals a Ferredoxin Fold with Specific Metal Binding Sites." J. Virol. 80, 10419-10427 (2006).
Assembly members:
wsv230, polymer, 85 residues, Formula weight is not available
Natural source: Common Name: SHRIMP WHITE SPOT SYNDROME VIRUS Taxonomy ID: 342409 Superkingdom: Viruses Kingdom: not available Genus/species: Whispovirus White spot syndrome virus 1
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
wsv230: GSHMATFQTDADFLLVGDDT
SRYEEVMKTFDTVEAVRKSD
LDDRVYMVCLKQGSTFVLNG
GIEELRLLTGDSTLEIQPMI
VPTTE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 240 |
| 15N chemical shifts | 80 |
| 1H chemical shifts | 507 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | wsv230 | 1 |
Entities:
Entity 1, wsv230 85 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | ALA | THR | PHE | GLN | THR | ASP | ||||
| 2 | ALA | ASP | PHE | LEU | LEU | VAL | GLY | ASP | ASP | THR | ||||
| 3 | SER | ARG | TYR | GLU | GLU | VAL | MET | LYS | THR | PHE | ||||
| 4 | ASP | THR | VAL | GLU | ALA | VAL | ARG | LYS | SER | ASP | ||||
| 5 | LEU | ASP | ASP | ARG | VAL | TYR | MET | VAL | CYS | LEU | ||||
| 6 | LYS | GLN | GLY | SER | THR | PHE | VAL | LEU | ASN | GLY | ||||
| 7 | GLY | ILE | GLU | GLU | LEU | ARG | LEU | LEU | THR | GLY | ||||
| 8 | ASP | SER | THR | LEU | GLU | ILE | GLN | PRO | MET | ILE | ||||
| 9 | VAL | PRO | THR | THR | GLU |
Samples:
sample_1: wsv230 3 mM; phosphate buffer 20 mM; H2O 90%; D2O 10%
sample_cond_1: ionic strength: 20 mM; pH: 6.72; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HSQC | not available | not available | not available |
Software:
NMRPipe v2004 - processing
CYANA v2.1 - refinement, structure solution
NMRView v5.2.2 - data analysis
NMR spectrometers:
- Bruker DRX 500 MHz
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