BMRB Entry 7323
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7323
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Title: 1H, 13C and 15N nmr chemical shift assignments for the colicin immunity protein IM2 PubMed: 20479265
Deposition date: 2006-10-24 Original release date: 2011-05-27
Authors: Boetzel, Ruth; Morel, Bertrand; Macdonald, Colin; Le Duff, Cecile; Spronk, Chris; Bonvin, Alexandre; James, Richard; Kleanthous, Colin; Moore, Geoffrey
Citation: Meenana, Nicola; Sharmaa, Amit; Fleishmanb, Sarel; MacDonaldc, Colin; Morelc, Bertrand; Boetzelc, Ruth; Moorec, Geoffrey; Bakerb, David; Kleanthousa, Colin. "The structural and energetic basis for high selectivity in a high-affinity protein-protein interaction" Proc. Natl. Acad. Sci. U S A. 107, 10080-10085.
Assembly members:
IM2, polymer, 86 residues, 9995 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichica coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
IM2: MELKHSISDYTEAEFLEFVK
KICRAEGATEEDDNKLVREF
ERLTEHPDGSDLIYYPRDDR
EDSPEGIVKEIKEWRAANGK
SGFKQG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 301 |
| 15N chemical shifts | 85 |
| 1H chemical shifts | 588 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Colicin immunity protein IM2 | 1 |
Entities:
Entity 1, Colicin immunity protein IM2 86 residues - 9995 Da.
| 1 | MET | GLU | LEU | LYS | HIS | SER | ILE | SER | ASP | TYR | ||||
| 2 | THR | GLU | ALA | GLU | PHE | LEU | GLU | PHE | VAL | LYS | ||||
| 3 | LYS | ILE | CYS | ARG | ALA | GLU | GLY | ALA | THR | GLU | ||||
| 4 | GLU | ASP | ASP | ASN | LYS | LEU | VAL | ARG | GLU | PHE | ||||
| 5 | GLU | ARG | LEU | THR | GLU | HIS | PRO | ASP | GLY | SER | ||||
| 6 | ASP | LEU | ILE | TYR | TYR | PRO | ARG | ASP | ASP | ARG | ||||
| 7 | GLU | ASP | SER | PRO | GLU | GLY | ILE | VAL | LYS | GLU | ||||
| 8 | ILE | LYS | GLU | TRP | ARG | ALA | ALA | ASN | GLY | LYS | ||||
| 9 | SER | GLY | PHE | LYS | GLN | GLY |
Samples:
sample_1: IM2, [U-100% 13C; U-100% 15N], 1.8 mM; Sodium Azide 14 mM; DTT 5 mM; Potassium Phosphate 50 mM
conditions_1: pH: 6.2; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | sample_1 | not available | conditions_1 |
| CBCA(CO)NH | sample_1 | not available | conditions_1 |
| HBHA(CBCACO)NH | sample_1 | not available | conditions_1 |
| HCCH-TOCSY | sample_1 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
| HN(CA)HA | sample_1 | not available | conditions_1 |
| 15N_NOESYHSQC | sample_1 | not available | conditions_1 |
| 13C_NOESYHSQC | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Varian Inova 600 MHz
- Varian Inova 800 MHz
Related Database Links:
| PDB | |
| EMBL | CAA25610 CAA26146 |
| GB | AAA23069 AAN28374 ABI95491 ACH81154 EDV85355 |
| REF | WP_000420691 WP_000420692 WP_000420693 WP_024222287 WP_032311204 |
| SP | P04482 |
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