BMRB Entry 7327
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7327
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Title: Homonuclear NMR Assignment of the Pheromone En-2 PubMed: 17701553
Deposition date: 2006-11-06 Original release date: 2007-09-17
Authors: Placzek, William; Etezady-Esfarjani, Touraj; Herrmann, Torsten; Peti, Wolfgang; Wuthrich, Kurt
Citation: Placzek, William; Etezady-Esfarjani, Touraj; Herrmann, Torsten; Pedrini, Bill; Peti, Wolfgang; Alimenti, Claudio; Luporini, Pierangelo; Wuthrich, Kurt. "Cold-adapted signal proteins: NMR structures of pheromones from the Antarctic ciliate Euplotes nobilii." IUBMB Life 59, 578-585 (2007).
Assembly members:
En-2, polymer, 60 residues, Formula weight is not available
Natural source: Common Name: Euplotes nobilii Taxonomy ID: 184062 Superkingdom: Eukaryota Kingdom: Alveolata Genus/species: Euplotes nobilii
Experimental source: Production method: Purified from the natural source
Entity Sequences (FASTA):
En-2: DIEDFYTSETCPYKNDSQLA
WDTCSGGTGNCGTVCCGQCF
SFPVSQSCAGMADSNDCPNA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 66 |
| 1H chemical shifts | 323 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | En-2 monomer | 1 |
Entities:
Entity 1, En-2 monomer 60 residues - Formula weight is not available
| 1 | ASP | ILE | GLU | ASP | PHE | TYR | THR | SER | GLU | THR | |
| 2 | CYS | PRO | TYR | LYS | ASN | ASP | SER | GLN | LEU | ALA | |
| 3 | TRP | ASP | THR | CYS | SER | GLY | GLY | THR | GLY | ASN | |
| 4 | CYS | GLY | THR | VAL | CYS | CYS | GLY | GLN | CYS | PHE | |
| 5 | SER | PHE | PRO | VAL | SER | GLN | SER | CYS | ALA | GLY | |
| 6 | MET | ALA | ASP | SER | ASN | ASP | CYS | PRO | ASN | ALA |
Samples:
sample_1: En-2 0.9 ± 0.05 mM; Sodium Phosphate 20 mM
conditions_1: ionic strength: 20 mM; pH: 6.0
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D TOCSY | sample_1 | not available | conditions_1 |
| 2D COSY | sample_1 | not available | conditions_1 |
| 2D natural abundance [13C, 1H]- HSQC | sample_1 | not available | conditions_1 |
| 2D NOESY | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 900 MHz