BMRB Entry 7419
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR7419
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Title: SOLUTION STRUCTURE OF AMINO TERMINAL DOMAIN OF HUMAN DNA POLYMERASE EPSILON SUBUNIT B REVEALS ITS HOMOLOGY TO C-DOMAINS OF AAA PROTEINS. PubMed: 18676977
Deposition date: 2008-03-06 Original release date: 2008-08-22
Authors: Nuutinen, T.; Fredriksson, K.; Tossavainen, H.; Pospiech, H.; Pirila, P.; Permi, P.; Annila, A.; Syvaoja, J.
Citation: Nuutinen, Tarmo; Tossavainen, Helena; Fredriksson, Kai; Pirila, Paivi; Permi, Perttu; Pospiech, Helmut; Syvaoja, Juhani. "The solution structure of the amino-terminal domain of human DNA polymerase epsilon subunit B is homologous to C-domains of AAA+ proteins" Nucleic Acids Res. 36, 5102-5110 (2008).
Assembly members:
AAA_protein, polymer, 99 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
AAA_protein: MGSSHHHHHHSQDPNSSSAR
LQVDMAPERLRSRALSAFKL
RGLLLRGEAIKYLTEALQSI
SELELEDKLEKIINAVEKQP
LSSNMIERSVVEAAVQESS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 386 |
| 15N chemical shifts | 90 |
| 1H chemical shifts | 646 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AAA_protein | 1 |
Entities:
Entity 1, AAA_protein 99 residues - Formula weight is not available
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | GLN | ASP | PRO | ASN | SER | SER | SER | ALA | ARG | ||||
| 3 | LEU | GLN | VAL | ASP | MET | ALA | PRO | GLU | ARG | LEU | ||||
| 4 | ARG | SER | ARG | ALA | LEU | SER | ALA | PHE | LYS | LEU | ||||
| 5 | ARG | GLY | LEU | LEU | LEU | ARG | GLY | GLU | ALA | ILE | ||||
| 6 | LYS | TYR | LEU | THR | GLU | ALA | LEU | GLN | SER | ILE | ||||
| 7 | SER | GLU | LEU | GLU | LEU | GLU | ASP | LYS | LEU | GLU | ||||
| 8 | LYS | ILE | ILE | ASN | ALA | VAL | GLU | LYS | GLN | PRO | ||||
| 9 | LEU | SER | SER | ASN | MET | ILE | GLU | ARG | SER | VAL | ||||
| 10 | VAL | GLU | ALA | ALA | VAL | GLN | GLU | SER | SER |
Samples:
sample: entity_1 mM
sample_conditions_1: pH: 6.62; pressure: 1.0 atm; temperature: 298.0 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | sample | isotropic | sample_conditions_1 |
| HNCACB | sample | isotropic | sample_conditions_1 |
| HNCOCACB | sample | isotropic | sample_conditions_1 |
| HNCO | sample | isotropic | sample_conditions_1 |
| HCCONH | sample | isotropic | sample_conditions_1 |
| CCCONH | sample | isotropic | sample_conditions_1 |
| HCCH-COSY | sample | isotropic | sample_conditions_1 |
| NOESY-HSQC | sample | isotropic | sample_conditions_1 |
Software:
CYANA - structure solution
AMBER v8 - structure solution
ProcheckNMR vNMR - structure solution
NMR spectrometers:
- VARIAN UNITYINOVA 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAG56707 BAJ21084 |
| GB | AAC39610 AAC51920 AAI12963 AAI26219 AAI26221 |
| REF | NP_001184259 NP_001184260 NP_002683 XP_003267698 XP_003267699 |
| SP | P56282 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
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