data_17972 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; U2/U6 Helix I ; _BMRB_accession_number 17972 _BMRB_flat_file_name bmr17972.str _Entry_type original _Submission_date 2011-10-03 _Accession_date 2011-10-03 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Burke Jordan E. . 2 Sashital Dipali G. . 3 Zuo Xiaobing E. . 4 Wang Yun-Xing . . 5 Butcher Samuel E. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 2 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 167 "13C chemical shifts" 80 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2012-04-04 update BMRB 'update entry citation' 2012-02-20 original author 'original release' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Structure of the yeast U2/U6 snRNA complex.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 22328579 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Burke Jordan E. . 2 Sashital Dipali G. . 3 Zuo Xiaobing . . 4 Wang Yun-Xing . . 5 Butcher Samuel E. . stop_ _Journal_abbreviation RNA _Journal_name_full 'RNA (New York, N.Y.)' _Journal_volume 18 _Journal_issue 4 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 673 _Page_last 683 _Year 2012 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name U2/U6snRNA _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label U2_U6snRNA $U2_U6snRNA stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_U2_U6snRNA _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class RNA _Name_common RNA_(5'-R(*GP*GP*CP*UP*UP*AP*GP*AP*UP*CP*AP*GP*AP*AP*AP*UP*GP*AP*UP*CP*AP*GP*CP*C)-3')_ _Molecular_mass 7716.717 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 24 _Mol_residue_sequence ; GGCUUAGAUCAGAAAUGAUC AGCC ; loop_ _Residue_seq_code _Residue_label 1 G 2 G 3 C 4 U 5 U 6 A 7 G 8 A 9 U 10 C 11 A 12 G 13 A 14 A 15 A 16 U 17 G 18 A 19 U 20 C 21 A 22 G 23 C 24 C stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $U2_U6snRNA 'baker's yeast' 4932 Eukaryota Fungi Saccharomyces cerevisiae stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_type _Vector_name $U2_U6snRNA 'cell free synthesis' . E. "coli - cell free" . N/A N/A stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $U2_U6snRNA . mM 0.5 0.6 'natural abundance' H2O 90 % . . 'natural abundance' D2O 10 % . . 'natural abundance' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $U2_U6snRNA . mM 0.5 0.6 'natural abundance' D2O 100 % . . 'natural abundance' stop_ save_ save_sample_3 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $U2_U6snRNA 0.3 mM '[U-98% 13C; U-98% 15N]' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ save_sample_4 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $U2_U6snRNA 0.3 mM '[U-98% 13C; U-98% 15N]' D2O 100 % 'natural abundance' stop_ save_ save_sample_5 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $U2_U6snRNA 0.3 mM '[U-98% 13C; U-98% 15N]' 'Pf1 phage' 17 mg/ml 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ save_sample_6 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $U2_U6snRNA 0.3 mM '[U-98% 13C; U-98% 15N]' 'Pf1 phage' 17 mg/ml 'natural abundance' D2O 100 % 'natural abundance' stop_ save_ save_sample_7 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $U2_U6snRNA . mM 0.5 0.6 'natural abundance' 'Pf1 phage' 18 mg/ml . . 'natural abundance' D2O 100 % . . 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_CNS _Saveframe_category software _Name CNS _Version 1.1 loop_ _Vendor _Address _Electronic_address 'Brunger, Adams, Clore, Gros, Nilges and Read' . . stop_ loop_ _Task 'structure solution' stop_ _Details . save_ save_xwinnmr _Saveframe_category software _Name xwinnmr _Version 3.5 loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection processing stop_ _Details . save_ save_VNMRJ _Saveframe_category software _Name VNMRJ _Version 1.1 loop_ _Vendor _Address _Electronic_address Varian . . stop_ loop_ _Task collection stop_ _Details . save_ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version 97.027.12.56 loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_SPARKY _Saveframe_category software _Name SPARKY _Version 3.114 loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'chemical shift assignment' 'data analysis' 'peak picking' stop_ _Details . save_ save_X-PLOR_NIH _Saveframe_category software _Name 'X-PLOR NIH' _Version 2.21 loop_ _Vendor _Address _Electronic_address 'Schwieters, Kuszewski, Tjandra and Clore' . . stop_ loop_ _Task refinement stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DMX _Field_strength 750 _Details Telemark save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 600 _Details Jaulan save_ ############################# # NMR applied experiments # ############################# save_2D_1H-1H_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_2D_1H-1H_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_2 save_ save_2D_1H-1H_TOCSY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H TOCSY' _Sample_label $sample_2 save_ save_2D_1H-13C_HSQC_aromatic_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC aromatic' _Sample_label $sample_2 save_ save_2D_1H-13C_HSQC_aliphatic_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC aliphatic' _Sample_label $sample_2 save_ save_2D_1H-15N_HMQC_6 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HMQC' _Sample_label $sample_3 save_ save_2D_1H-13C_HSQC_aromatic_7 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC aromatic' _Sample_label $sample_4 save_ save_2D_1H-13C_HSQC_aliphatic_8 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC aliphatic' _Sample_label $sample_4 save_ save_2D_1H-15N_HMQC_9 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HMQC' _Sample_label $sample_5 save_ save_2D_1H-13C_HSQC_aromatic_10 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC aromatic' _Sample_label $sample_6 save_ save_2D_1H-13C_HSQC_aliphatic_11 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC aliphatic' _Sample_label $sample_6 save_ save_2D_HNN-COSY_12 _Saveframe_category NMR_applied_experiment _Experiment_name '2D HNN-COSY' _Sample_label $sample_3 save_ save_3D_HCCH-TOCSY_13 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HCCH-TOCSY' _Sample_label $sample_4 save_ save_3D_HCCH-COSY_14 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HCCH-COSY' _Sample_label $sample_4 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details 'pH achieved by addition of HCl or NaOH' loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 15 5 mM pH 7.0 0.1 pH pressure 1 . atm temperature 283 . K stop_ save_ save_sample_conditions_2 _Saveframe_category sample_conditions _Details 'pH achieved by addition of HCl or NaOH' loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 15 5 mM pH 7.0 .01 pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-1H NOESY' '2D 1H-1H TOCSY' '2D 1H-13C HSQC aromatic' '2D 1H-13C HSQC aliphatic' stop_ loop_ _Sample_label $sample_2 stop_ _Sample_conditions_label $sample_conditions_2 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name U2_U6snRNA _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 G H1' H 5.812 0.005 1 2 1 1 G H2' H 4.933 0.002 2 3 1 1 G H3' H 4.745 0.002 1 4 1 1 G H8 H 8.133 0.002 1 5 1 1 G C2' C 74.991 0.016 1 6 1 1 G C3' C 75.078 0.016 1 7 1 1 G C8 C 139.203 0.016 1 8 2 2 G H1' H 5.909 0.001 1 9 2 2 G H2' H 4.625 0.001 2 10 2 2 G H3' H 4.518 0.003 1 11 2 2 G H8 H 7.623 0.004 1 12 2 2 G C1' C 92.971 0.016 1 13 2 2 G C2' C 76.533 0.016 1 14 2 2 G C8 C 137.017 0.016 1 15 3 3 C H1' H 5.579 0.003 1 16 3 3 C H2' H 4.378 0.007 2 17 3 3 C H3' H 4.521 0.004 1 18 3 3 C H5 H 5.258 0.004 1 19 3 3 C H5'' H 4.082 0.001 2 20 3 3 C H6 H 7.521 0.003 1 21 3 3 C C1' C 94.051 0.016 1 22 3 3 C C2' C 72.524 0.016 1 23 3 3 C C6 C 141.032 0.016 1 24 4 4 U H1' H 5.773 0.004 1 25 4 4 U H2' H 4.231 0.001 1 26 4 4 U H3' H 4.234 0.002 1 27 4 4 U H5 H 5.529 0.005 1 28 4 4 U H6 H 7.733 0.004 1 29 4 4 U C1' C 92.153 0.016 1 30 4 4 U C3' C 75.971 0.016 1 31 4 4 U C6 C 142.351 0.016 1 32 5 5 U H1' H 5.829 0.005 1 33 5 5 U H2' H 4.268 0.008 1 34 5 5 U H3' H 4.624 0.005 1 35 5 5 U H4' H 4.521 0.006 1 36 5 5 U H5 H 5.755 0.008 1 37 5 5 U H6 H 7.701 0.005 1 38 5 5 U C1' C 92.378 0.016 1 39 5 5 U C2' C 75.488 0.016 1 40 5 5 U C3' C 75.452 0.016 1 41 5 5 U C6 C 143.715 0.016 1 42 6 6 A H1' H 5.990 0.005 1 43 6 6 A H2 H 8.059 0.003 1 44 6 6 A H2' H 4.810 0.003 2 45 6 6 A H3' H 4.748 0.001 1 46 6 6 A H4' H 4.560 0.006 1 47 6 6 A H8 H 8.294 0.001 1 48 6 6 A C1' C 93.269 0.016 1 49 6 6 A C2 C 155.161 0.016 1 50 6 6 A C2' C 75.812 0.016 1 51 6 6 A C3' C 75.960 0.016 1 52 6 6 A C8 C 141.857 0.016 1 53 7 7 G H1' H 5.553 0.005 1 54 7 7 G H2' H 4.775 0.001 2 55 7 7 G H3' H 4.630 0.006 1 56 7 7 G H5' H 4.232 0.003 2 57 7 7 G H8 H 7.775 0.001 1 58 7 7 G C1' C 91.697 0.016 1 59 7 7 G C2' C 75.112 0.016 1 60 7 7 G C8 C 137.796 0.016 1 61 8 8 A H1' H 5.994 0.001 1 62 8 8 A H2 H 7.787 0.004 1 63 8 8 A H2' H 4.575 0.007 2 64 8 8 A H8 H 7.872 0.002 1 65 8 8 A C1' C 90.733 0.016 1 66 8 8 A C2 C 153.894 0.016 1 67 8 8 A C2' C 74.021 0.016 1 68 8 8 A C8 C 140.196 0.016 1 69 9 9 U H1' H 5.508 0.002 1 70 9 9 U H2' H 4.338 0.002 1 71 9 9 U H3' H 4.438 0.006 1 72 9 9 U H5 H 4.983 0.002 1 73 9 9 U H5' H 4.563 0.006 2 74 9 9 U H5'' H 4.537 0.006 2 75 9 9 U H6 H 7.634 0.005 1 76 9 9 U C1' C 93.503 0.016 1 77 9 9 U C2' C 75.634 0.016 1 78 9 9 U C3' C 72.204 0.016 1 79 9 9 U C6 C 141.341 0.016 1 80 10 10 C H1' H 5.537 0.004 1 81 10 10 C H2' H 4.379 0.003 2 82 10 10 C H3' H 4.549 0.006 1 83 10 10 C H4' H 4.428 0.005 1 84 10 10 C H5 H 5.590 0.001 1 85 10 10 C H6 H 7.798 0.007 1 86 10 10 C C1' C 93.744 0.016 1 87 10 10 C C3' C 72.608 0.016 1 88 10 10 C C6 C 141.416 0.016 1 89 11 11 A H1' H 5.879 0.001 1 90 11 11 A H2 H 7.287 0.001 1 91 11 11 A H2' H 4.512 0.001 2 92 11 11 A H3' H 4.662 0.003 1 93 11 11 A H8 H 7.895 0.014 1 94 11 11 A C1' C 92.987 0.016 1 95 11 11 A C2 C 153.099 0.016 1 96 11 11 A C3' C 72.432 0.016 1 97 11 11 A C8 C 139.276 0.016 1 98 12 12 G H1' H 5.371 0.004 1 99 12 12 G H2' H 4.333 0.001 2 100 12 12 G H3' H 4.570 0.006 1 101 12 12 G H5'' H 4.069 0.001 2 102 12 12 G H8 H 7.175 0.001 1 103 12 12 G C1' C 92.427 0.016 1 104 12 12 G C8 C 136.426 0.016 1 105 13 13 A H1' H 5.687 0.004 1 106 13 13 A H2 H 7.812 0.006 1 107 13 13 A H2' H 4.682 0.002 2 108 13 13 A H3' H 4.416 0.003 1 109 13 13 A H4' H 4.205 0.002 1 110 13 13 A H8 H 8.302 0.002 1 111 13 13 A C1' C 91.468 0.016 1 112 13 13 A C2 C 154.388 0.016 1 113 13 13 A C2' C 75.879 0.016 1 114 13 13 A C8 C 142.135 0.016 1 115 14 14 A H1' H 5.455 0.004 1 116 14 14 A H2 H 8.148 0.002 1 117 14 14 A H2' H 4.352 0.008 2 118 14 14 A H8 H 7.865 0.011 1 119 14 14 A C1' C 92.012 0.016 1 120 14 14 A C2 C 155.470 0.016 1 121 14 14 A C8 C 140.067 0.016 1 122 15 15 A H1' H 6.024 0.004 1 123 15 15 A H2 H 7.692 0.005 1 124 15 15 A H2' H 4.454 0.010 2 125 15 15 A H3' H 4.983 0.005 1 126 15 15 A H4' H 4.448 0.006 1 127 15 15 A H5' H 4.521 0.006 2 128 15 15 A H8 H 8.062 0.003 1 129 15 15 A C1' C 91.892 0.016 1 130 15 15 A C2 C 154.326 0.016 1 131 15 15 A C8 C 140.751 0.016 1 132 16 16 U H1' H 3.873 0.016 1 133 16 16 U H2' H 4.421 0.002 1 134 16 16 U H3' H 4.183 0.004 1 135 16 16 U H5 H 5.885 0.004 1 136 16 16 U H5' H 4.221 0.006 2 137 16 16 U H6 H 7.828 0.005 1 138 16 16 U C1' C 94.307 0.016 1 139 16 16 U C2' C 74.467 0.016 1 140 16 16 U C3' C 74.427 0.016 1 141 16 16 U C6 C 142.477 0.016 1 142 17 17 G H1' H 5.752 0.001 1 143 17 17 G H2' H 4.584 0.002 2 144 17 17 G H3' H 4.609 0.002 1 145 17 17 G H5'' H 4.075 0.006 2 146 17 17 G H8 H 7.696 0.022 1 147 17 17 G C1' C 92.442 0.016 1 148 17 17 G C2' C 75.442 0.016 1 149 17 17 G C3' C 72.918 0.016 1 150 17 17 G C8 C 136.689 0.016 1 151 18 18 A H1' H 5.842 0.003 1 152 18 18 A H2 H 7.647 0.004 1 153 18 18 A H2' H 4.399 0.001 2 154 18 18 A H3' H 4.534 0.002 1 155 18 18 A H4' H 4.477 0.006 1 156 18 18 A H8 H 7.748 0.004 1 157 18 18 A C1' C 93.096 0.016 1 158 18 18 A C2 C 153.739 0.016 1 159 18 18 A C3' C 74.631 0.016 1 160 18 18 A C8 C 139.298 0.016 1 161 19 19 U H1' H 5.465 0.004 1 162 19 19 U H2' H 4.334 0.008 1 163 19 19 U H5 H 4.942 0.001 1 164 19 19 U H6 H 7.515 0.004 1 165 19 19 U C1' C 93.224 0.016 1 166 19 19 U C6 C 141.218 0.016 1 167 20 20 C H1' H 5.614 0.001 1 168 20 20 C H2' H 4.466 0.004 2 169 20 20 C H3' H 4.370 0.006 1 170 20 20 C H4' H 4.416 0.006 1 171 20 20 C H5 H 5.474 0.003 1 172 20 20 C H5' H 4.086 0.006 2 173 20 20 C H6 H 7.604 0.005 1 174 20 20 C C1' C 93.736 0.016 1 175 20 20 C C2' C 76.679 0.016 1 176 20 20 C C6 C 141.249 0.016 1 177 21 21 A H1' H 5.829 0.004 1 178 21 21 A H2 H 7.280 0.003 1 179 21 21 A H2' H 4.628 0.002 2 180 21 21 A H3' H 4.279 0.006 1 181 21 21 A H4' H 4.416 0.006 1 182 21 21 A H5' H 4.167 0.006 2 183 21 21 A H8 H 8.047 0.004 1 184 21 21 A C1' C 90.753 0.016 1 185 21 21 A C2 C 153.565 0.016 1 186 21 21 A C8 C 140.163 0.016 1 187 22 22 G H1' H 5.445 0.001 1 188 22 22 G H2' H 4.441 0.003 2 189 22 22 G H3' H 4.404 0.002 1 190 22 22 G H4' H 4.285 0.003 1 191 22 22 G H5'' H 4.068 0.006 2 192 22 22 G H8 H 7.333 0.001 1 193 22 22 G C1' C 92.900 0.016 1 194 22 22 G C2' C 74.854 0.016 1 195 22 22 G C8 C 136.333 0.016 1 196 23 23 C H1' H 5.542 0.001 1 197 23 23 C H2' H 4.254 0.002 2 198 23 23 C H5 H 5.187 0.002 1 199 23 23 C H6 H 7.635 0.003 1 200 23 23 C C1' C 94.101 0.016 1 201 23 23 C C6 C 141.372 0.016 1 202 24 24 C H1' H 5.782 0.005 1 203 24 24 C H2' H 4.009 0.002 2 204 24 24 C H3' H 4.175 0.001 1 205 24 24 C H5 H 5.528 0.003 1 206 24 24 C H6 H 7.672 0.013 1 207 24 24 C C1' C 92.900 0.016 1 208 24 24 C C2' C 77.547 0.016 1 209 24 24 C C3' C 69.714 0.016 1 210 24 24 C C6 C 141.838 0.016 1 stop_ save_ save_assigned_chem_shift_2ist_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-1H NOESY' '2D 1H-15N HMQC' stop_ loop_ _Sample_label $sample_1 $sample_5 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name U2_U6snRNA _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 G H1 H 12.728 0.004 1 2 1 1 G H21 H 8.235 0.001 2 3 1 1 G H22 H 6.914 0.001 2 4 2 2 G H1 H 13.254 0.001 1 5 2 2 G H21 H 8.447 0.005 2 6 2 2 G H22 H 6.813 0.017 2 7 3 3 C H5 H 5.101 0.020 1 8 4 4 U H1' H 5.647 0.020 1 9 4 4 U H5 H 5.403 0.020 1 10 4 4 U H6 H 7.624 0.020 1 11 7 7 G H1 H 11.847 0.001 1 12 8 8 A H2 H 7.654 0.007 1 13 8 8 A H61 H 7.784 0.020 2 14 8 8 A H62 H 6.636 0.020 2 15 9 9 U H3 H 13.989 0.035 1 16 10 10 C H1' H 5.487 0.080 1 17 10 10 C H41 H 8.122 0.002 2 18 10 10 C H42 H 6.821 0.001 2 19 11 11 A H2 H 7.126 0.001 1 20 11 11 A H2' H 4.354 0.020 1 21 12 12 G H1 H 10.105 0.002 1 22 12 12 G H2' H 4.181 0.020 1 23 15 15 A H2 H 7.509 0.020 1 24 15 15 A H62 H 7.842 0.020 2 25 16 16 U H3 H 13.208 0.036 1 26 17 17 G H1 H 11.542 0.001 1 27 17 17 G H21 H 7.775 0.020 2 28 17 17 G H22 H 6.002 0.005 2 29 18 18 A H2 H 7.499 0.004 1 30 19 19 U H3 H 13.849 0.031 1 31 20 20 C H41 H 7.845 0.017 2 32 20 20 C H42 H 6.688 0.008 2 33 21 21 A H1' H 5.690 0.020 1 34 21 21 A H61 H 7.753 0.020 2 35 22 22 G H1 H 13.188 0.005 1 36 22 22 G H21 H 8.354 0.001 2 37 24 24 C H1' H 5.636 0.020 1 stop_ save_