# 1 "/opt/topspin2.1.4/exp/stan/nmr/lists/pp/user/noesyhsqcetgp3d.nus.old"
# 1 "C:/Bruker/TOPSPIN2.1.4/exp/stan/nmr/lists/pp/user/noesyhsqcetgp3d.nus.old"
;noesyhsqcetgp3d
;avance-version (07/04/04)
;NOESY-HSQC
;3D sequence with
;   homonuclear correlation via dipolar coupling 
;   dipolar coupling may be due to noe or chemical exchange.
;   H-1/X correlation via double inept transfer
;phase sensitive (t1)
;phase sensitive using Echo/Antiecho-TPPI gradient selection (t2)
;using trim pulses in inept transfer
;with decoupling during acquisition
;using shaped pulses for inversion on f2 - channel
;(use parameterset NOESYHSQCETGP3D)
;
;A.L. Davis, J. Keeler, E.D. Laue & D. Moskau, J. Magn. Reson. 98, 
;   207-216 (1992)
;
;$CLASS=HighRes
;$DIM=3D
;$TYPE=
;$SUBTYPE=
;$COMMENT=


# 1 "C:/Bruker/TOPSPIN2.1.4/exp/stan/nmr/lists/pp/Avance.incl" 1
;Avance2.incl
;   for 1
;
;avance-version (07/08/21)
;
;$CLASS=HighRes Incl
;$COMMENT=





































































































































;$Id: Avance2.incl,v 1.17.2.1 2007/09/14 16:17:35 ber Exp $
# 24 "C:/Bruker/TOPSPIN2.1.4/exp/stan/nmr/lists/pp/user/noesyhsqcetgp3d.nus.old" 2


# 1 "C:/Bruker/TOPSPIN2.1.4/exp/stan/nmr/lists/pp/Grad.incl" 1
;Grad2.incl  -  include file for Gradient Spectroscopy
;   for 1
;
;avance-version (07/01/17)
;
;$CLASS=HighRes Incl
;$COMMENT=



















define list<gradient> EA=<EA>



;$Id: Grad2.incl,v 1.12 2007/01/22 14:22:35 ber Exp $
# 25 "C:/Bruker/TOPSPIN2.1.4/exp/stan/nmr/lists/pp/user/noesyhsqcetgp3d.nus.old" 2


# 1 "C:/Bruker/TOPSPIN2.1.4/exp/stan/nmr/lists/pp/Delay.incl" 1
;Delay.incl  -  include file for commonly used delays
;
;version 00/02/07
;
;$CLASS=HighRes Incl
;$COMMENT=


;general delays

define delay DELTA
define delay DELTA1
define delay DELTA2
define delay DELTA3
define delay DELTA4
define delay DELTA5
define delay DELTA6
define delay DELTA7
define delay DELTA8

define delay TAU
define delay TAU1
define delay TAU2
define delay TAU3
define delay TAU4
define delay TAU5


;delays for centering pulses

define delay CEN_HN1
define delay CEN_HN2
define delay CEN_HN3
define delay CEN_HC1
define delay CEN_HC2
define delay CEN_HC3
define delay CEN_HC4
define delay CEN_HP1
define delay CEN_HP2
define delay CEN_CN1
define delay CEN_CN2
define delay CEN_CN3
define delay CEN_CN4
define delay CEN_CP1
define delay CEN_CP2


;loop counters

define loopcounter COUNTER
define loopcounter SCALEF
define loopcounter FACTOR1
define loopcounter FACTOR2
define loopcounter FACTOR3



;$Id: Delay.incl,v 1.12 2005/11/10 12:16:58 ber Exp $
# 26 "C:/Bruker/TOPSPIN2.1.4/exp/stan/nmr/lists/pp/user/noesyhsqcetgp3d.nus.old" 2



;********Added for NUS*******
define delay short
"short=50u"
"l2=td2/2"
"l3=td1/2"
;****************************
"in0=inf1/2"
"in10=inf2/2"


"p2=p1*2"

"p4=p3*2"

"d0=3u"

"d4=1s/(cnst2*4)"

"d10=3u"

"d11=30m"

"d12=20u"


"DELTA2=d4-larger(p2,p14)/2-p16-8u"
"DELTA3=d4-larger(p2,p14)/2"


"DELTA=larger(p14,p22)+d0*2"
"DELTA1=p16+d16+larger(p2,p22)+d10*2"






aqseq 321


# 1 "mc_line 69 file C:/Bruker/TOPSPIN2.1.4/exp/stan/nmr/lists/pp/user/noesyhsqcetgp3d.nus.old dc-measurement inserted automatically"
    dccorr
# 68 "C:/Bruker/TOPSPIN2.1.4/exp/stan/nmr/lists/pp/user/noesyhsqcetgp3d.nus.old"

# 1 "mc_line 1 file /opt/topspin2.1.4/exp/stan/nmr/lists/pp/user/noesyhsqcetgp3d.nus.old dc-measurement inserted automatically"
    dccorr
# 1 "/opt/topspin2.1.4/exp/stan/nmr/lists/pp/user/noesyhsqcetgp3d.nus.old"
1 ze
	d11 do:f2
2	d11 do:f2
	short*5
3	short*6
4	5u
if "vd < 2u" goto 100
	short*5
5	short*5
6	d11 pl12:f2
 d1 do:f2
 d12 pl0:f2
  (p1 ph7)
  DELTA
  (p2 ph8)
  d0


  (center (p14:sp3 ph1):f2 (p22 ph1):f3 )




  d0
  (p1 ph1):f1
  d8
  (p1 ph1):f1
  DELTA3 pl0:f2
  4u
  (center (p2 ph1) (p14:sp3 ph6):f2 )
  4u
  DELTA3 pl2:f2 setnmr3|0 setnmr0|34|32|33
 ; p28 ph1
  4u
  (p1 ph2) (p3 ph3):f2
  d10 


  (center (p2 ph5) (p22 ph1):f3 )




  d10
  p16:gp1*EA
  d16
  (p4 ph4):f2
  DELTA1
  (ralign (p1 ph1) (p3 ph4):f2 )
  DELTA3 pl0:f2
  (center (p2 ph1) (p14:sp3 ph1):f2 )
  4u
  p16:gp2
  DELTA2 pl12:f2
  4u setnmr3^0 setnmr0^34^32^33
  go=2 ph31 cpd2:f2
;**************************************************
	d11 do:f2 wr #0 if #0 zd
	short*5 igrad EA
lo to 3 times 2
	short ip3*2
	short ip6*2
	short*2 ip31*2
	short id10
	1u ivd
	goto 200

100	short ip3*2
	short ip6*2
	short*2 ip31*2
	short id10
	1u ivd

200	5u
lo to 4 times l2
	short rd10
	short ip7
	short ip8
	short rp3
	short rp6
	short rp31
lo to 5 times 2
	short id0
	short*4 ip31*2
lo to 6 times l3
1m

;  d1 do:f2 mc #0 to 2
;     F1PH(rd10 & ip7 & ip8, id0)
;     F2EA(igrad EA, id10 & ip3*2 & ip6*2 & ip31*2)
exit
   

ph1=0 
ph2=1
ph3=0 2
ph4=0 0 0 0 0 0 0 0 2 2 2 2 2 2 2 2
ph5=0 0 2 2
ph6=0
ph7=0 0 0 0 2 2 2 2
ph8=1 1 1 1 3 3 3 3
ph31=0 2 0 2 2 0 2 0 2 0 2 0 0 2 0 2


;pl0 : 120dB
;pl1 : f1 channel - power level for pulse (default)
;pl2 : f2 channel - power level for pulse (default)
;pl3 : f3 channel - power level for pulse (default)
;pl12: f2 channel - power level for CPD/BB decoupling
;sp3: f2 channel - shaped pulse 180 degree
;p1 : f1 channel -  90 degree high power pulse
;p2 : f1 channel - 180 degree high power pulse
;p3 : f2 channel -  90 degree high power pulse
;p4 : f2 channel - 180 degree high power pulse
;p14: f2 channel - 180 degree shaped pulse for inversion
;p16: homospoil/gradient pulse
;p22: f3 channel - 180 degree high power pulse
;p28: f1 channel - trim pulse
;d0 : incremented delay (F1 in 3D)                     [3 usec]
;d1 : relaxation delay; 1-5 * T1
;d4 : 1/(4J)XH
;d8 : mixing time
;d10: incremented delay (F2 in 3D)                     [3 usec]
;d11: delay for disk I/O                             [30 msec]
;d12: delay for power switching                      [20 usec]
;d16: delay for homospoil/gradient recovery
;cnst2: = J(XH)
;in0: 1/(2 * SW(H)) =  DW(H)
;nd0: 2
;in10: 1/(2 * SW(X)) = DW(X)
;nd10: 2
;NS: 8 * n
;DS: >= 16
;td1: number of experiments in F1
;td2: number of experiments in F2
;FnMODE: States-TPPI (or TPPI) in F1
;FnMODE: echo-antiecho in F2
;cpd2: decoupling according to sequence defined by cpdprg2
;pcpd2: f2 channel - 90 degree pulse for decoupling sequence


;use gradient ratio:    gp 1 : gp 2
;                         80 : 20.1    for C-13
;                         80 :  8.1    for N-15

;for z-only gradients:
;gpz1: 80%
;gpz2: 20.1% for C-13, 8.1% for N-15

;use gradient files:   
;gpnam1: SINE.100
;gpnam2: SINE.100


                                          ;preprocessor-flags-start
;1: for C-13 and N-15 labeled samples start experiment with 
;             option -DLABEL_CN (eda: ZGOPTNS)
                                          ;preprocessor-flags-end



;$Id: noesyhsqcetgp3d,v 1.3 2004/03/09 16:08:59 ber Exp $