# 1 "/u/exp/stan/nmr/lists/pp/nnoehsqc_arg.gc" ; ; water-flip-back NOESY-HSQC ; ; programed by Junji Iwahara ; ; modified by JI on 12/2/04 ; ; implemented @ dexter by GC 10/2009 # 1 "/u/exp/stan/nmr/lists/pp//Avance.incl" ;Avance.incl ; ;version 99/08/02 # 11 "/u/exp/stan/nmr/lists/pp/nnoehsqc_arg.gc" # 1 "/u/exp/stan/nmr/lists/pp//Grad.incl" ;Grad.incl - include file for Gradient Spectroscopy ;avance-version ;version 99/02/19 define list EA= # 12 "/u/exp/stan/nmr/lists/pp/nnoehsqc_arg.gc" # 1 "/u/exp/stan/nmr/lists/pp//Delay.incl" ;Delay.incl - include file for commonly used delays ;avance-version ;version 99/02/19 ;general delays define delay DELTA define delay DELTA1 define delay DELTA2 define delay DELTA3 define delay DELTA4 define delay DELTA5 define delay DELTA6 define delay DELTA7 define delay DELTA8 define delay TAU define delay TAU1 define delay TAU2 define delay TAU3 define delay TAU4 define delay TAU5 ;delays for centering pulses define delay CEN_HN1 define delay CEN_HN2 define delay CEN_HN3 define delay CEN_HC1 define delay CEN_HC2 define delay CEN_HC3 define delay CEN_HC4 define delay CEN_CN1 define delay CEN_CN2 ;loop counters define loopcounter COUNTER define loopcounter SCALEF define loopcounter FACTOR1 define loopcounter FACTOR2 define loopcounter FACTOR3 # 13 "/u/exp/stan/nmr/lists/pp/nnoehsqc_arg.gc" ;#define ONE_D ;#define CARBON ; PFG length "p18=3m" "p15=2.5m" "p16=1.0m" "p17=400u" ; rect. 180 "p2=p1*2.0" "p14=p13*2.0" ; transfer delay "d2=2.25m" ;defined parameters. "d21=d2-p22*0.5-p17-100u" "d24=d2-p17-p5-125u" ;centering "CEN_HN2=(p22-p2)/2" "CEN_HN1=p5+p1+20u-p22*0.5" ; NOE mixing "d7=d8-p18-p21-1.6m" ; d8 = (NOE mixing time) ;initial delay "d0=in0*0.5-p1*0.637" ; for 1H (135, -180) # 48 "/u/exp/stan/nmr/lists/pp/nnoehsqc_arg.gc" "d10=in10*0.5-p21*0.637-p1" ; for 15N (90,-180) ;pulse sequence. 30m 1 ze 2 60m do:f2 3 30m 4 30m 5 30m 6 20u do:f2 20u pl1:f1 20u pl13:f3 20u pl12:f2 20u fq3:f3 ; CA -> 56ppm d1 4u cpd2:f2 # 70 "/u/exp/stan/nmr/lists/pp/nnoehsqc_arg.gc" 4u (p1 ph4):f1 d0 d0 (p1 ph17):f1 5u do:f3 ; NOE mixing start 5u do:f2 85u setnmr2|0 setnmr0|34 d7 pl2:f2 (p21 ph0):f2 ; kill 15N-steady state mag. 5u ; p18:gp1 ; 1.5m ; NOE mixing end (p1 ph0):f1 5u ; p17:gp4 ; 95u ; d21 (CEN_HN2 p2 ph1):f1 (p22:sp4 ph10):f2 d21 5u ; p17:gp4 ; 95u ; (p1 ph1):f1 5u 20u pl0:f1 (p5:sp5 ph5:r):f1 5u ; p15:gp1 ; 95u ; 20u pl1:f1 (p21 ph11):f2 d10 # 113 "/u/exp/stan/nmr/lists/pp/nnoehsqc_arg.gc" (p2 ph0):f1 d10 (p21 ph12):f2 5u ; p16:gp2 ; 95u ; 20u pl0:f1 ; set 120 dB (p9:sp9 ph7:r):f1 ; half-gaussian pulse (90) 5u 20u pl1:f1 (p1 ph0):f1 5u 5u ; p17:gp3 ; 95u ; d24 pl0:f1 (CEN_HN1 p22:sp4 ph10):f2 (p5:sp5 ph8:r 20u p2:sp2 ph3 20u p5:sp5 ph8:r):f1 d24 pl12:f2 5u ; p17:gp3 ; 95u ; 5u setnmr2^0 setnmr0^34 go=2 ph31 cpds2:f2 45m do:f2 wr #0 if #0 zd 15m ip4 lo to 3 times 2 15m id0 15m ip31*2 lo to 4 times l1 15m rd0 15m ip11 lo to 5 times 2 15m id10 15m ip31*2 lo to 6 times l2 30m setnmr2^0 setnmr0^34 exit ;-- 1H pulse -- ph0=0 ph1=1 ph3=2 ph4=0 2 ph17=(8)1 ;-- Water-selective pulse -- ph5=0 ph7=2 ph8=0 ;-- 15N pulses -- ph10=0 ph11=0 0 2 2 ph12=0 ;-- phases for receiver -- ph31=0 2 2 0